Invitrogen

Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (44-682G) in ICC/IF

Immunofluorescent analysis of JNK1/2 (pT183/pT185) was done on 70% confluent log phase A549 cells treated with Anisomycin (25 µg/mL for 30 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JNK1/2 (pT183/pT185) Rabbit polyclonal Antibody (Product # 44-68... View More

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody in Western Blot (WB)

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody in Immunohistochemistry (IHC)

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody

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Product Details

44-682G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 52 publications 52 publications

Immunohistochemistry (IHC)

Assay-dependent

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Insect, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JNK1&2 that contains threonine 183 and tyrosine 185. This region is conserved among many species including mouse, rat, chicken, nematode, fruit fly, and in JNK3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533720

Product Specific Information

This antibody is reactive to human and rat JNK1&2. Other species of JNK1&2 have not been tested, and JNK3 (found primarily in neuronal cell lines) has not been detected. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK1&2. The final product is generated by affinity chromatography using a JNK1&2-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. Positive controls used: HEK 293 +/- UV irradiation treatment; PC12 cells +/- sorbitol.

Target Information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Prochlorperazine-Induced Decrease in Autonomous Muscle Activity during Hindlimb Unloading Is Accompanied by Preserved Slow Myosin mRNA Expression.

Current issues in molecular biology

Sharlo KA,Lvova ID,Tyganov SA,Sergeeva KV,Kalashnikov VY,Kalashnikova EP,Mirzoev TM,Kalamkarov GR,Shevchenko TF,Shenkman BS

44-682G was used in Western Blotting to find that prochlorperazine blocks slow-to-fast fiber-type transformation in disused skeletal muscles of rats, possibly through affecting calcium and ROS-related signaling.

Fri Jun 30 00:00:00 UTC 2023

Curative Effect of AD-MSCs against Cisplatin-Induced Hepatotoxicity in Rats is Potentiated by Azilsartan: Targeting Oxidative Stress, MAPK, and Apoptosis Signaling Pathways.

Stem cells international

Bekhit AA,Beshay ON,Fawzy MA,Abdel-Hafez SMN,Batiha GE,Ataya FS,Fathy M

44-682G was used in Western Blot to demonstrate that AZIL improves the hepatoprotective potential of AD-MSCs against CISP-induced hepatotoxicity by modulating oxidative stress, mitogen-activated protein kinase, and apoptotic pathways.

Thu Nov 02 00:00:00 UTC 2023

Electroacupuncture Attenuates Fibromyalgia Pain via Toll-like Receptor 4 in the Mouse Brain.

Life (Basel, Switzerland)

Lai PC,Yen CM,Lin MC,Chen YH,Liao HY,Huang YW,Lin YW

44-682G was used in Western Blotting to show that inflammation can activate the TLR4 pathway and provided new possible therapeutic targets for FM pain.

Thu May 11 00:00:00 UTC 2023

Repurposing gestrinone for tumor suppressor through P21 reduction regulated by JNK in gynecological cancer.

Translational research : the journal of laboratory and clinical medicine

Ciou HH,Lee TH,Wang HC,Ding YR,Tseng CJ,Wang PH,Tsai MH,Tzeng SL

44-682G was used in Western Blotting to prove that gestrinone has the potential to protect against cancer through regulation of the JNK-P21 axis.

Sun May 01 00:00:00 UTC 2022

Progesterone suppresses the progression of colonic carcinoma by increasing the activity of the GADD45α/JNK/c‑Jun signalling pathway.

Oncology reports

Zhang YL,Wen XD,Guo X,Huang SQ,Wang TT,Zhou PT,Li W,Zhou LF,Hu YH

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Western Blot

Tue Jun 01 00:00:00 UTC 2021

Phyllanthin inhibits MOLT-4 leukemic cancer cell growth and induces apoptosis through the inhibition of AKT and JNK signaling pathway.

Journal of biochemical and molecular toxicology

Wang H,Chinnathambi A,Alahmadi TA,Alharbi SA,Veeraraghavan VP,Krishna Mohan S,Hussain S,Ramamoorthy K,Rengarajan T

44-682G was used in Western Blot to determine the impact of phyllanthin on the MOLT-4 cytotoxic effect.

Tue Jun 01 00:00:00 UTC 2021

Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model.

International journal of molecular medicine

Inprasit C,Huang YC,Lin YW

44-682G was used in Western Blotting to show body weight maintenance may be modulated by calcium ion channel TRPV1.

Sun Mar 01 00:00:00 UTC 2020

Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model.

International journal of molecular medicine

Inprasit C,Huang YC,Lin YW

44-682G was used in Western Blotting to show body weight maintenance may be modulated by calcium ion channel TRPV1.

Sun Mar 01 00:00:00 UTC 2020

Airway epithelial specific deletion of Jun-N-terminal kinase 1 attenuates pulmonary fibrosis in two independent mouse models.

PloS one

van der Velden JL,Alcorn JF,Chapman DG,Lundblad LKA,Irvin CG,Davis RJ,Butnor K,Janssen-Heininger YMW

44-682G was used in Immunohistochemistry to demonstrate prominent activation of JNK in bronchial epithelia using the mouse models of bleomycin- or AdTGFβ1- induce fibrosis.

Mon Apr 06 00:00:00 UTC 2020

Airway epithelial specific deletion of Jun-N-terminal kinase 1 attenuates pulmonary fibrosis in two independent mouse models.

PloS one

van der Velden JL,Alcorn JF,Chapman DG,Lundblad LKA,Irvin CG,Davis RJ,Butnor K,Janssen-Heininger YMW

44-682G was used in Immunohistochemistry to demonstrate prominent activation of JNK in bronchial epithelia using the mouse models of bleomycin- or AdTGFβ1- induce fibrosis.

Mon Apr 06 00:00:00 UTC 2020

Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway.

International journal of inflammation

Fang K,Kevil CG

44-682G was used in Western Blot to investigate modulation of VEGF and JNK signalling pathways in endothelial colon tissue for treatment of different diseases.

Wed Jan 12 00:00:00 UTC 2022

Caveolin-1 Scaffolding Domain Peptide Regulates Colon Endothelial Cell Survival through JNK Pathway.

International journal of inflammation

Fang K,Kevil CG

44-682G was used in Western Blot to investigate modulation of VEGF and JNK signalling pathways in endothelial colon tissue for treatment of different diseases.

Wed Jan 12 00:00:00 UTC 2022

Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing.

Nature

Mahmoudi S,Mancini E,Xu L,Moore A,Jahanbani F,Hebestreit K,Srinivasan R,Li X,Devarajan K,Prélot L,Ang CE,Shibuya Y,Benayoun BA,Chang ALS,Wernig M,Wysocka J,Longaker MT,Snyder MP,Brunet A

44-682G was used in Western Blotting to show that this variability may reflect distinct stochastic ageing trajectories between individuals, and could help in developing personalized strategies to improve iPS cell generation and wound healing in elderly individuals.

Tue Oct 01 00:00:00 UTC 2019

Glucosamine improves survival in a mouse model of sepsis and attenuates sepsis-induced lung injury and inflammation.

The Journal of biological chemistry

Hwang JS,Kim KH,Park J,Kim SM,Cho H,Lee Y,Han IO

44-682G was used in Western Blotting to investigate the effects of glucosamine on septic lethality and sepsis-induced inflammation using animal models of mice and zebrafish.

Fri Jan 11 00:00:00 UTC 2019

Inhibition of JNK signaling in the Asian malaria vector Anopheles stephensi extends mosquito longevity and improves resistance to Plasmodium falciparum infection.

PLoS pathogens

Souvannaseng L,Hun LV,Baker H,Klyver JM,Wang B,Pakpour N,Bridgewater JM,Napoli E,Giulivi C,Riehle MA,Luckhart S

44-682G was used in Western Blotting to assess the effects of c-Jun N terminal kinase signalling manipulations on Anopheles stephensi fecundity, lifespan, intermediary metabolism, and Plasmodium falciparum development.

Thu Nov 01 00:00:00 UTC 2018

Gastric corticotropin-releasing factor influences mast cell infiltration in a rat model of functional dyspepsia.

PloS one

Hagiwara SI,Kaushal E,Paruthiyil S,Pasricha PJ,Hasdemir B,Bhargava A

44-682G was used in Western Blotting to delineate the role of the corticotropin-releasing factor system in the pathogenesis of functional dyspepsia in a rat model.

Fri Feb 22 00:00:00 UTC 2019

Rapid Activation of Transforming Growth Factor β-Activated Kinase 1 in Chondrocytes by Phosphorylation and K⁶³ -Linked Polyubiquitination Upon Injury to Animal Articular Cartilage.

Arthritis & rheumatology (Hoboken, N.J.)

Ismail HM,Didangelos A,Vincent TL,Saklatvala J

44682G was used in western blot to demonstrate that injury results in TAK1 activation by phosphorylation and K63-linked polyubiquitination

Wed Mar 01 00:00:00 UTC 2017

Rapid Activation of Transforming Growth Factor β-Activated Kinase 1 in Chondrocytes by Phosphorylation and K⁶³ -Linked Polyubiquitination Upon Injury to Animal Articular Cartilage.

Arthritis & rheumatology (Hoboken, N.J.)

Ismail HM,Didangelos A,Vincent TL,Saklatvala J

44682G was used in western blot to demonstrate that injury results in TAK1 activation by phosphorylation and K63-linked polyubiquitination

Wed Mar 01 00:00:00 UTC 2017

Abscisic acid induces a transient shift in signaling that enhances NF-κB-mediated parasite killing in the midgut of Anopheles stephensi without reducing lifespan or fecundity.

Parasites & vectors

Glennon EKK,Torrevillas BK,Morrissey SF,Ejercito JM,Luckhart S

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Western Blot

Thu Jul 13 00:00:00 UTC 2017

Lipopolysaccharide (LPS)-stimulated iNOS Induction Is Increased by Glucosamine under Normal Glucose Conditions but Is Inhibited by Glucosamine under High Glucose Conditions in Macrophage Cells.

The Journal of biological chemistry

Hwang JS,Kwon MY,Kim KH,Lee Y,Lyoo IK,Kim JE,Oh ES,Han IO

44-682G was used in Western Blotting to show that glucosamine regulates inflammation by sensing energy states of normal and fuel excess.

Fri Feb 03 00:00:00 UTC 2017

Brief Report: JNK-2 Controls Aggrecan Degradation in Murine Articular Cartilage and the Development of Experimental Osteoarthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Ismail HM,Miotla-Zarebska J,Troeberg L,Tang X,Stott B,Yamamoto K,Nagase H,Fosang AJ,Vincent TL,Saklatvala J

44-682G was used in western blot to analyze development of experimental osteoarthritis and aggrecan degradation in murine articular cartilage by JNK-2

Sun May 01 00:00:00 UTC 2016

The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes.

EMBO reports

Afonina IS,Van Nuffel E,Baudelet G,Driege Y,Kreike M,Staal J,Beyaert R

44682G was used in western blot to show that CARD14 activates p38 and JNK MAP kinase pathways

Wed Jun 01 00:00:00 UTC 2016

NADPH Oxidases Are Essential for Macrophage Differentiation.

The Journal of biological chemistry

Xu Q,Choksi S,Qu J,Jang J,Choe M,Banfi B,Engelhardt JF,Liu ZG

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Western Blot

Fri Sep 16 00:00:00 UTC 2016

The tumour suppressor CYLD regulates the p53 DNA damage response.

Nature communications

Fernández-Majada V,Welz PS,Ermolaeva MA,Schell M,Adam A,Dietlein F,Komander D,Büttner R,Thomas RK,Schumacher B,Pasparakis M

44-682G was used in western blot elucidate the mechanisms by which CYLD suppresses tumors

Fri Aug 26 00:00:00 UTC 2016

Interleukin-1 Acts via the JNK-2 Signaling Pathway to Induce Aggrecan Degradation by Human Chondrocytes.

Arthritis & rheumatology (Hoboken, N.J.)

Ismail HM,Yamamoto K,Vincent TL,Nagase H,Troeberg L,Saklatvala J

44-682G was used in western blot to identify the intracellular signaling pathways by which IL- causes aggrecan degradation by human chondrocytes and investigate how aggrecanase activity is regulated in chondrocytes.

Wed Jul 01 00:00:00 UTC 2015

Upregulated RIP3 Expression Potentiates MLKL Phosphorylation-Mediated Programmed Necrosis in Toxic Epidermal Necrolysis.

The Journal of investigative dermatology

Kim SK,Kim WJ,Yoon JH,Ji JH,Morgan MJ,Cho H,Kim YC,Kim YS

44-682G was used in western blot to evaluate the role of RIP3 in toxic epidermal necrolysis.

Sat Aug 01 00:00:00 UTC 2015

Functional comparison of herpes simplex virus 1 (HSV-1) and HSV-2 ICP27 homologs reveals a role for ICP27 in virion release.

Journal of virology

Park D,Lalli J,Sedlackova-Slavikova L,Rice SA

44-682G was used in western blot to compare ICP27t2 and ICP27 from herpes simplex virus

Sun Mar 01 00:00:00 UTC 2015

RNF4 negatively regulates NF-κB signaling by down-regulating TAB2.

FEBS letters

Tan B,Mu R,Chang Y,Wang YB,Wu M,Tu HQ,Zhang YC,Guo SS,Qin XH,Li T,Li WH,Zhang XM,Li AL,Li HY

44-682G was used in western blot to identify negative regulators of NF-κB signaling.

Mon Sep 14 00:00:00 UTC 2015

Suppression of interferon β gene transcription by inhibitors of bromodomain and extra-terminal (BET) family members.

The Biochemical journal

Malik N,Vollmer S,Nanda SK,Lopez-Pelaez M,Prescott A,Gray N,Cohen P

44-682G was used in western blot to study inhibitors of bromodomain and extra-terminal (BET) family members by suppression of interferon beta gene transcription

Mon Jun 15 00:00:00 UTC 2015

A Novel ASK Inhibitor AGI-1067 Inhibits TLR-4-Mediated Activation of ASK1 by Preventing Dissociation of Thioredoxin from ASK1.

Cardiovascular pharmacology: open access

Zheng S,Long L,Li Y,Xu Y,Jiqin Z,Ji W,Min W

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Western Blot

Wed Apr 01 00:00:00 UTC 2015

Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis.

Oncotarget

Shi S,Wang Q,Xu J,Jang JH,Padilla MT,Nyunoya T,Xing C,Zhang L,Lin Y

44-682G was used in western blot to investigate if Chal-24 can be combined with cisplatin for better cancer therapy.

Fri Jan 30 00:00:00 UTC 2015

RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis.

Nature

Dannappel M,Vlantis K,Kumari S,Polykratis A,Kim C,Wachsmuth L,Eftychi C,Lin J,Corona T,Hermance N,Zelic M,Kirsch P,Basic M,Bleich A,Kelliher M,Pasparakis M

44-682G was used in western blot to show that RIPK1 regulates homeostasis and prevents inflammation in barrier tissues by inhibiting epithelial cell apoptosis and necroptosis

Thu Sep 04 00:00:00 UTC 2014

A JNK-mediated autophagy pathway that triggers c-IAP degradation and necroptosis for anticancer chemotherapy.

Oncogene

He W,Wang Q,Srinivasan B,Xu J,Padilla MT,Li Z,Wang X,Liu Y,Gou X,Shen HM,Xing C,Lin Y

44-682G was used in western blot to identify a novel anticancer mechanism that functions through autophagy-mediated necroptosis

Thu Jun 05 00:00:00 UTC 2014

Dual oxidase 2 in lung epithelia is essential for hyperoxia-induced acute lung injury in mice.

Antioxidants & redox signaling

Kim MJ,Ryu JC,Kwon Y,Lee S,Bae YS,Yoon JH,Ryu JH

44-682G was used in western blot to study ROS production induced by hyperoxia in type II alveolar epithelial cells and acute lung injury

Sat Nov 01 00:00:00 UTC 2014

Increased ERK and JNK activation and decreased ERK/JNK ratio are associated with long-term organ damage in patients with systemic lupus erythematosus.

Rheumatology (Oxford, England)

Bloch O,Amit-Vazina M,Yona E,Molad Y,Rapoport MJ

44-682G was used in western blot to test if extracellular signal-regulated kinase and c-Jun N-terminal kinase are associated with long-term organ damage in SLE patients.

Sun Jun 01 00:00:00 UTC 2014

SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter.

Journal of immunology (Baltimore, Md. : 1950)

Gürtler C,Carty M,Kearney J,Schattgen SA,Ding A,Fitzgerald KA,Bowie AG

44-682G was used in western blot to examine TLR-induced cytokine production by SARM-deficient murine macrophages

Thu May 15 00:00:00 UTC 2014

Retaining MKP1 expression and attenuating JNK-mediated apoptosis by RIP1 for cisplatin resistance through miR-940 inhibition.

Oncotarget

Wang Q,Shi S,He W,Padilla MT,Zhang L,Wang X,Zhang B,Lin Y

44-682G was used in western blot to study the role of receptor-interacting protein 1 in cancer's response to chemotherapy

Sat Mar 15 00:00:00 UTC 2014

JNK1 controls dendritic field size in L2/3 and L5 of the motor cortex, constrains soma size, and influences fine motor coordination.

Frontiers in cellular neuroscience

Komulainen E,Zdrojewska J,Freemantle E,Mohammad H,Kulesskaya N,Deshpande P,Marchisella F,Mysore R,Hollos P,Michelsen KA,Mågard M,Rauvala H,James P,Coffey ET

44-682G was used in immunocytochemistry to investigate the role of JNK-1 signaling in prevalent dendrite disorders

Mon Oct 13 00:00:00 UTC 2014

Supplementation with α-lipoic acid, CoQ10, and vitamin E augments running performance and mitochondrial function in female mice.

PloS one

Abadi A,Crane JD,Ogborn D,Hettinga B,Akhtar M,Stokl A,Macneil L,Safdar A,Tarnopolsky M

44-682G was used in western blot to assess the effects of an antioxidant cocktail (α-lipoic acid, vitamin E and coenzyme Q0) on exercise performance, muscle function, and training adaptations in mice.

Mon Sep 23 00:00:00 UTC 2013

Attenuation of TNFSF10/TRAIL-induced apoptosis by an autophagic survival pathway involving TRAF2- and RIPK1/RIP1-mediated MAPK8/JNK activation.

Autophagy

He W,Wang Q,Xu J,Xu X,Padilla MT,Ren G,Gou X,Lin Y

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Western Blot

Sat Dec 01 00:00:00 UTC 2012

p38γ and p38δ kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.

Proceedings of the National Academy of Sciences of the United States of America

Risco A,del Fresno C,Mambol A,Alsina-Beauchamp D,MacKenzie KF,Yang HT,Barber DF,Morcelle C,Arthur JS,Ley SC,Ardavin C,Cuenda A

Tue Jul 10 00:00:00 UTC 2012

p38γ and p38δ kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.

Proceedings of the National Academy of Sciences of the United States of America

Risco A,del Fresno C,Mambol A,Alsina-Beauchamp D,MacKenzie KF,Yang HT,Barber DF,Morcelle C,Arthur JS,Ley SC,Ardavin C,Cuenda A

Tue Jul 10 00:00:00 UTC 2012

The death domain-containing protein Unc5CL is a novel MyD88-independent activator of the pro-inflammatory IRAK signaling cascade.

Cell death and differentiation

Heinz LX,Rebsamen M,Rossi DC,Staehli F,Schroder K,Quadroni M,Gross O,Schneider P,Tschopp J

44-682G was used in western blot to study the role of Unc5CL in the pro-inflammatory IRAK signaling cascade

Sun Apr 01 00:00:00 UTC 2012

Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling.

BMC immunology

Smeets RL,Fleuren WW,He X,Vink PM,Wijnands F,Gorecka M,Klop H,Bauerschmidt S,Garritsen A,Koenen HJ,Joosten I,Boots AM,Alkema W

Wed Mar 14 00:00:00 UTC 2012

T-cell receptor-induced JNK activation requires proteolytic inactivation of CYLD by MALT1.

The EMBO journal

Staal J,Driege Y,Bekaert T,Demeyer A,Muyllaert D,Van Damme P,Gevaert K,Beyaert R

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Western Blot

Wed May 04 00:00:00 UTC 2011

zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC-MAPKs-AP-1 pathway.

Cell death and differentiation

Wu YT,Tan HL,Huang Q,Sun XJ,Zhu X,Shen HM

44-682G was used in western blot to study the role of TNF in zVAD-induced necroptosis in L929 cells

Sat Jan 01 00:00:00 UTC 2011

Extracellular HMGB1 released by NMDA treatment confers neuronal apoptosis via RAGE-p38 MAPK/ERK signaling pathway.

Neurotoxicity research

Kim SW,Lim CM,Kim JB,Shin JH,Lee S,Lee M,Lee JK

44-682G was used in western blot to examine the endogenous danger molecule-like function of high mobility group box 1

Mon Aug 01 00:00:00 UTC 2011

Reactive oxygen species control senescence-associated matrix metalloproteinase-1 through c-Jun-N-terminal kinase.

Journal of cellular physiology

Dasgupta J,Kar S,Liu R,Joseph J,Kalyanaraman B,Remington SJ,Chen C,Melendez JA

44-682G was used in western blot to report the redox-responsive molecular signals that drive senescence-associated matrix metalloproteinase-1 expression

Fri Oct 01 00:00:00 UTC 2010

Reactive oxygen species control senescence-associated matrix metalloproteinase-1 through c-Jun-N-terminal kinase.

Journal of cellular physiology

Dasgupta J,Kar S,Liu R,Joseph J,Kalyanaraman B,Remington SJ,Chen C,Melendez JA

44-682G was used in western blot to report the redox-responsive molecular signals that drive senescence-associated matrix metalloproteinase-1 expression

Fri Oct 01 00:00:00 UTC 2010

Growth-stimulatory effect of resveratrol in human cancer cells.

Molecular carcinogenesis

Fukui M,Yamabe N,Kang KS,Zhu BT

44-682G was used in western blot to demonstrate that resveratrol promotes the growth of certain human cancer cells

Sun Aug 01 00:00:00 UTC 2010

Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis.

Nature genetics

Karner CM,Chirumamilla R,Aoki S,Igarashi P,Wallingford JB,Carroll TJ

44-682G was used in western blot to study the contribution of Wnt9b signaling to kidney morphogenesis

Wed Jul 01 00:00:00 UTC 2009

Apoptosis commitment and activation of mitochondrial Bax during anoikis is regulated by p38MAPK.

Cell death and differentiation

Owens TW,Valentijn AJ,Upton JP,Keeble J,Zhang L,Lindsay J,Zouq NK,Gilmore AP

44-682G was used in western blot to identify p38MAPK as an activator of Bax

Sun Nov 01 00:00:00 UTC 2009

Herpes simplex virus type 1 ICP27 induces p38 mitogen-activated protein kinase signaling and apoptosis in HeLa cells.

Journal of virology

Gillis PA,Okagaki LH,Rice SA

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Immunofluorescence

Sun Feb 01 00:00:00 UTC 2009

Herpes simplex virus type 1 ICP27 induces p38 mitogen-activated protein kinase signaling and apoptosis in HeLa cells.

Journal of virology

Gillis PA,Okagaki LH,Rice SA

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Immunofluorescence

Sun Feb 01 00:00:00 UTC 2009

Age-dependent increases in interstitial collagenase and MAP Kinase levels are exacerbated by superoxide dismutase deficiencies.

Experimental gerontology

Dasgupta J,Kar S,Van Remmen H,Melendez JA

44-682G was used in western blot to investigate the contribution of reactive oxygen species to the age-dependent increase in collagenase

Sat Aug 01 00:00:00 UTC 2009

The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.

Nature immunology

Pobezinskaya YL,Kim YS,Choksi S,Morgan MJ,Li T,Liu C,Liu Z

44-682G was used in western blot to report how TRADD regulates tumor necrosis factor receptor 1 signaling

Mon Sep 01 00:00:00 UTC 2008

The proteolytic activity of the paracaspase MALT1 is key in T cell activation.

Nature immunology

Rebeaud F,Hailfinger S,Posevitz-Fejfar A,Tapernoux M,Moser R,Rueda D,Gaide O,Guzzardi M,Iancu EM,Rufer N,Fasel N,Thome M

44-682G was used in western blot to investigate the function of MALT1 proteolytic activity

Sat Mar 01 00:00:00 UTC 2008

Critical role of c-jun (NH2) terminal kinase in paracetamol- induced acute liver failure.

Gut

Henderson NC,Pollock KJ,Frew J,Mackinnon AC,Flavell RA,Davis RJ,Sethi T,Simpson KJ

Sun Jul 01 00:00:00 UTC 2007

Critical role of c-jun (NH2) terminal kinase in paracetamol- induced acute liver failure.

Gut

Henderson NC,Pollock KJ,Frew J,Mackinnon AC,Flavell RA,Davis RJ,Sethi T,Simpson KJ

Sun Jul 01 00:00:00 UTC 2007

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway.

Journal of virology

Zapata HJ,Nakatsugawa M,Moffat JF

44-682G was used in western blot to study the effect of infection with varicella-zoster virus on c-Jun N-terminal kinase signaling

Mon Jan 01 00:00:00 UTC 2007

Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.

Journal of leukocyte biology

Ratthé C,Pelletier M,Chiasson S,Girard D

Tue May 01 00:00:00 UTC 2007

Response to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) differs in mouse strains and reveals a divergence in JNK signaling and COX-2 induction prior to loss of neurons in the substantia nigra pars compacta.

Brain research

Boyd JD,Jang H,Shepherd KR,Faherty C,Slack S,Jiao Y,Smeyne RJ

44-682G was used in Western Blotting to provide evidence toward a mechanism of strain-dependent sensitivity to the neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

Wed Oct 17 00:00:00 UTC 2007

Prosurvival and proapoptotic intracellular signaling in rat spiral ganglion neurons in vivo after the loss of hair cells.

The Journal of comparative neurology

Alam SA,Robinson BK,Huang J,Green SH

Published figure using Phospho-JNK1/JNK2 (Thr183, Tyr185) polyclonal antibody (Product # 44-682G) in Immunofluorescence

Mon Aug 20 00:00:00 UTC 2007

The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.

Gynecologic oncology

Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R

Fri Sep 01 00:00:00 UTC 2006

Homozygous deletion of MKK4 in ovarian serous carcinoma.

Cancer biology & therapy

Nakayama K,Nakayama N,Davidson B,Katabuchi H,Kurman RJ,Velculescu VE,Shih IeM,Wang TL

44-682G was used in immunohistochemistry - paraffin section to search for chromosomal deletions in ovarian serous carcinoma and identify genes that could contribute to disease

Thu Jun 01 00:00:00 UTC 2006

JSAP1 is required for the cell adhesion and spreading of mouse embryonic fibroblasts.

Biochemical and biophysical research communications

Chae HJ,Ha HY,Im JY,Song JY,Park S,Han PL

44-682G was used in western blot to investigate the roles of JSAP1 and JIP1 in cell adhesion and spreading

Fri Jun 30 00:00:00 UTC 2006

Matrix metalloproteinases (MMP), EMMPRIN (extracellular matrix metalloproteinase inducer) and mitogen-activated protein kinases (MAPK): co-expression in metastatic serous ovarian carcinoma.

Clinical & experimental metastasis

Davidson B,Givant-Horwitz V,Lazarovici P,Risberg B,Nesland JM,Trope CG,Schaefer E,Reich R

Thu Dec 18 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; JNK 55; JNK-46; JNK-55; JNK/SAPK alpha; JNK1 beta1 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; Mitogen-activated protein kinase 9; OTTHUMP00000019552; p54 gamma; p54-alpha; Phospho-JNK; PRKM; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK 1; SAPK gamma; SAPK-alpha; SAPK1a; SAPK1c; stress activated protein kinase alpha II; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2

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Gene Aliases: AI849689; AI851083; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK8; MAPK9; p54a; p54aSAPK; PRKM8; PRKM9; SAPK; SAPK1; SAPK1A; SAPK1C

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UniProt ID: (Human) P45983, (Human) P45984, (Rat) P49185, (Mouse) Q91Y86, (Rat) P49186, (Mouse) Q9WTU6

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Entrez Gene ID: (Human) 5599, (Human) 5601, (Rat) 116554, (Mouse) 26419, (Rat) 50658, (Mouse) 26420

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Function(s)

protein serine/threonine kinase activity JUN kinase activity protein binding ATP binding kinase activity enzyme binding histone deacetylase regulator activity histone deacetylase binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process mitogen-activated protein kinase kinase kinase binding nucleotide binding protein kinase activity MAP kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

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Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (44-682G) in ICC/IF

Product Details

44-682G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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