Invitrogen

Phospho-JNK1/JNK2 (Thr183, Tyr185) Recombinant Rabbit Monoclonal Antibody (D12H7L17)

Advanced Verification
4 Published Figures
13 References
View all (11) JNK1/JNK2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

700031

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-1 µg/mL
View 11 publications 11 publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

4-6 µg/mL
-

Flow Cytometry (Flow)

0.5-5 µg/1x10^6 cells
-

ELISA (ELISA)

1-5 µg/mL
-

ChIP assay (ChIP)

1 µL
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Insect, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

D12H7L17

Immunogen

A peptide corresponding to amino acids 179-189 and 180-189 of P45983 and P45984, respectively.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532273

Product Specific Information

This antibody is predicted to react with mouse, rat, primate, canine, bovine, porcine, chicken, equine, zebrafish and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; JNK 55; JNK-46; JNK-55; JNK/SAPK alpha; JNK1 beta1 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; Mitogen-activated protein kinase 9; OTTHUMP00000019552; Phospho-JNK; PRKM; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK 1; SAPK1a; SAPK1c; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2

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Gene Aliases: AI849689; AI851083; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK8; MAPK9; p54a; p54aSAPK; PRKM8; PRKM9; SAPK; SAPK1; SAPK1A; SAPK1C

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UniProt ID: (Human) P45983, (Human) P45984, (Mouse) Q91Y86, (Mouse) Q9WTU6

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Entrez Gene ID: (Human) 5599, (Human) 5601, (Mouse) 26419, (Mouse) 26420

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Function(s)
protein serine/threonine kinase activity JUN kinase activity protein binding ATP binding kinase activity enzyme binding histone deacetylase regulator activity histone deacetylase binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process mitogen-activated protein kinase kinase kinase binding nucleotide binding protein kinase activity MAP kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
release of cytochrome c from mitochondria positive regulation of protein phosphorylation protein phosphorylation protein targeting to mitochondrion activation of cysteine-type endopeptidase activity involved in apoptotic process response to stress JNK cascade JUN phosphorylation central nervous system development response to mechanical stimulus response to toxic substance positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cell morphogenesis involved in differentiation response to amine neuron projection development positive regulation of prostaglandin biosynthetic process regulation of protein ubiquitination positive regulation of prostaglandin secretion positive regulation of chemokine production cellular response to UV Fc-epsilon receptor signaling pathway response to drug regulation of circadian rhythm positive regulation of nitric oxide biosynthetic process positive regulation of transcription, DNA-templated regulation of JNK cascade response to cadmium ion rhythmic process regulation of sequence-specific DNA binding transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to growth factor stimulus positive regulation of podosome assembly positive regulation of apoptotic signaling pathway response to UV regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of histone deacetylation positive regulation of cyclase activity negative regulation of protein binding regulation of protein localization positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of protein metabolic process cellular response to mechanical stimulus positive regulation of deacetylase activity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway ossification regulation of transcription, DNA-templated inflammatory response response to osmotic stress signal transduction response to heat regulation of gene expression phosphorylation positive regulation of cell migration positive regulation of microtubule polymerization response to hydrogen peroxide positive regulation of DNA replication determination of dorsal identity cellular response to hydrogen peroxide cellular response to nitric oxide type B pancreatic cell apoptotic process neuronal stem cell population maintenance programmed necrotic cell death positive regulation of determination of dorsal identity positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
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