Invitrogen

Phospho-LCK/FYN (Tyr416) Polyclonal Antibody

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-110156

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
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Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around Y416 of human SRC Family (NP_0054081)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2855567

Product Specific Information

Immunogen sequence: NEYTA

Target Information

LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found. Fyn is a membrane-associated non-receptor protein tyrosine kinase that belongs to the Src-family of kinases. The unique N-terminal domain of Fyn interacts with the CD3 and Delta chains of the TcR. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-syn protooncogene; FYN oncogene related to SRC, FGR, YES; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OKT3-induced calcium influx regulator; p56(LSTRA) protein-tyrosine kinase; p56-LCK; p59-Fyn; Protein YT16; Proto-oncogene c-Fyn; Proto-oncogene Lck; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase LCK; SLK; Src-like kinase; src/yes-related novel; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine kinase p59fyn(T); Tyrosine-protein kinase Fyn; Tyrosine-protein kinase Lck

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Gene Aliases: FYN; IMD22; LCK; LSK; p56lck; p59-FYN; pp58lck; SLK; SYN; YT16

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UniProt ID: (Human) P06241, (Human) P06239

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Entrez Gene ID: (Human) 2534, (Human) 3932

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Function(s)
G-protein coupled receptor binding glycoprotein binding protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding tubulin binding T cell receptor binding CD4 receptor binding CD8 receptor binding phosphatidylinositol 3-kinase binding ion channel binding metal ion binding ephrin receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity peptide hormone receptor binding growth factor receptor binding protein serine/threonine phosphatase activity protein C-terminus binding kinase activity protein kinase binding protein phosphatase binding SH2 domain binding identical protein binding ATPase binding
Process(es)
MAPK cascade neuron migration stimulatory C-type lectin receptor signaling pathway adaptive immune response protein phosphorylation calcium ion transport transmembrane receptor protein tyrosine kinase signaling pathway axon guidance central nervous system development blood coagulation learning feeding behavior regulation of cell shape negative regulation of gene expression positive regulation of neuron projection development regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling viral process cell migration peptidyl-tyrosine phosphorylation cell differentiation platelet activation forebrain development T cell costimulation negative regulation of protein ubiquitination intracellular signal transduction cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis T cell activation regulation of cell proliferation negative regulation of protein catabolic process response to drug positive regulation of tyrosine phosphorylation of Stat5 protein regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neuron apoptotic process positive regulation of GTPase activity innate immune response response to ethanol phosphatidylinositol phosphorylation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling dendrite morphogenesis regulation of defense response to virus by virus activated T cell proliferation T cell receptor signaling pathway leukocyte migration detection of mechanical stimulus involved in sensory perception of pain cellular response to peptide hormone stimulus cellular response to transforming growth factor beta stimulus positive regulation of protein localization to nucleus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand protein dephosphorylation cellular zinc ion homeostasis activation of cysteine-type endopeptidase activity involved in apoptotic process hemopoiesis T cell differentiation B cell receptor signaling pathway positive regulation of T cell receptor signaling pathway positive regulation of T cell activation release of sequestered calcium ion into cytosol regulation of lymphocyte activation positive regulation of intrinsic apoptotic signaling pathway
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