Invitrogen

Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Polyclonal Antibody

Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Antibody in Western Blot (WB)

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Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Antibody (PA5-17495) in WB

Western blot analysis of Phospho-MARK Family in extracts from Raji and BaF3 cells showing endogenous levels of phosphorylated MARK family members using Phospho-MARK Family polyclonal antibody (Product # PA5-17495).

Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Antibody in Immunohistochemistry (IHC)

Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Antibody

Product Image Gallery

Product Details

PA5-17495

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding threonine 215 of human MARK1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

59 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10980994

Product Specific Information

This antibody does not react with MARK4.

It is not recommended to aliquot this antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Henderson MX,Chung CH,Riddle DM,Zhang B,Gathagan RJ,Seeholzer SH,Trojanowski JQ,Lee VMY

PA5-17495 was used in Immunocytochemistry to support a role for autophagy as a survival mechanism in response to stress due to ribosomal P proteins protein deficiency.

Wed Jun 14 00:00:00 UTC 2017

Membrane-binding and activation of LKB1 by phosphatidic acid is essential for development and tumour suppression.

Nature communications

Dogliotti G,Kullmann L,Dhumale P,Thiele C,Panichkina O,Mendl G,Houben R,Haferkamp S,Püschel AW,Krahn MP

PA5-17495 was used in Western Blot to show that targeting of the kinase to the membrane by a direct binding of LKB1 to phosphatidic acid is essential to fully activate its kinase activity.

Mon Jun 26 00:00:00 UTC 2017

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

The Journal of biological chemistry

Wu Q,Sun M,Bernard LP,Zhang H

PA5-17495 was used in Western Blotting to reveal insight into the regulation of PSD-95 in dendritic spine structural plasticity through phosphorylation-mediated regulation of protein dynamics and conformation.

Fri Sep 29 00:00:00 UTC 2017

MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

PloS one

Bernard LP,Zhang H

PA5-17495 was used in western blot to show that NMDA receptors activate MARK/Par1 via protein kinase A in primary hippocampal neurons

Wed Jan 27 00:00:00 UTC 2016

MARK4 and MARK3 associate with early tau phosphorylation in Alzheimer's disease granulovacuolar degeneration bodies.

Acta neuropathologica communications

Lund H,Gustafsson E,Svensson A,Nilsson M,Berg M,Sunnemark D,von Euler G

PA5-17495 was used in immunohistochemistry to study the significance for Alzheimer's disease of the presence in granulovacuolar degradation bodies of MARK-3/MARK-4 and Ser262 phosphorylated tau

Mon Feb 17 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: C-TAK1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 1; ELKL motif kinase 2; ELKL motif serine-threonine protein kinase 3; ELKL motif serine/threonine-protein kinase 3; EMK-1; EMK-2; EMK1; EMK2/MARK3 serine-threonine protein kinase; MAP/microtubule affinity-regulating kinase 1; MAP/microtubule affinity-regulating kinase 2; MAP/microtubule affinity-regulating kinase 3; MARK1; mark1 {ECO:0000312|RGD:619882}; MARK2; mark2 {ECO:0000312|RGD:708483}; MPK-10; Par-1a; Par-1b; Par-1c; PAR1 homolog; PAR1 homolog b; PAR1 homolog c; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Ser/Thr protein kinase PAR-1B; serine/threonine kinase; serine/threonine protein kinase EMK; Serine/threonine-protein kinase MARK1; Serine/threonine-protein kinase MARK2; Serine/threonine-protein kinase p78; testicular tissue protein Li 117

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Gene Aliases: 1600015G02Rik; A430080F22Rik; AW491150; B930025N23Rik; C-TAK1; CTAK1; Emk; EMK-1; EMK1; EMK2; Emk3; ETK-1; ETK1; KIAA1477; KP78; MARK; MARK1; MARK2; MARK3; mKIAA1477; mPar-1c; MPK10; PAR-1; Par-1a; Par-1b; Par-1c; PAR1A; Par1b; Par1c

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UniProt ID: (Human) Q9P0L2, (Human) Q7KZI7, (Human) P27448, (Mouse) Q8VHJ5, (Rat) O08678, (Rat) O08679, (Mouse) Q05512, (Rat) Q8VHF0, (Mouse) Q03141

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Entrez Gene ID: (Human) 4139, (Human) 2011, (Human) 4140, (Mouse) 226778, (Rat) 117016, (Rat) 60328, (Mouse) 13728, (Rat) 170577, (Mouse) 17169

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Function(s)

magnesium ion binding phosphatidylserine binding protein serine/threonine kinase activity ATP binding phosphatidylinositol-4,5-bisphosphate binding tau-protein kinase activity phosphatidic acid binding protein binding lipid binding protein kinase activator activity poly(A) RNA binding cadherin binding involved in cell-cell adhesion nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding

Process(es)

neuron migration protein phosphorylation positive regulation of neuron projection development Wnt signaling pathway establishment of cell polarity intracellular signal transduction establishment or maintenance of epithelial cell apical/basal polarity protein autophosphorylation regulation of axonogenesis regulation of cytoskeleton organization microtubule cytoskeleton organization cytoskeleton organization multicellular organism development phosphorylation cell differentiation MAPK cascade mitophagy peptidyl-threonine phosphorylation activation of protein kinase activity mitochondrion localization cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Polyclonal Antibody

Phospho-MARK1/MARK2/MARK3 (Thr215, Thr208, Thr234) Antibody (PA5-17495) in WB

Product Details

PA5-17495

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Bioinformatics

Performance Guarantee

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