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Phospho-MARK2 (Thr595) Antibody (600-401-895) in WB
Western Blot of Rabbit anti-MARK2pT595 antibody. Lane 1: 293T cells untransfected. Lane 2: 293T cells transfected with MARK2 and PKC-zeta. Lane 3: 293T cells transfected with MARK2. Load: 35 µg per lane. Primary antibody: MARK2pT595 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/O... View More
Product Details
600-401-895
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
Target Information
MARK2 is a serine/threonine protein kinase involved in the control of cell polarity and microtubule stability. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ELKL motif kinase; ELKL motif kinase 1; EMK-1; EMK1; MAP/microtubule affinity-regulating kinase 2; MARK2; mark2 {ECO:0000312|RGD:708483}; MGC99619; OTTHUMP00000218338; OTTHUMP00000218339; OTTHUMP00000218340; OTTHUMP00000218343; Par-1b; PAR1 homolog; PAR1 homolog b; Ser/Thr protein kinase PAR-1B; serine/threonine kinase; serine/threonine protein kinase EMK; Serine/threonine-protein kinase MARK2; testicular tissue protein Li 117
Gene Aliases: BOS_24829; Emk; EMK-1; EMK1; MARK2; PAR-1; Par-1b; Par1b
UniProt ID: (Human) Q7KZI7, (Rat) O08679, (Mouse) Q05512
Entrez Gene ID: (Bovine) 535197, (Dog) 483769, (Human) 2011, (Rat) 60328, (Mouse) 13728
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
lipid binding
protein kinase activator activity
poly(A) RNA binding
tau-protein kinase activity
cadherin binding involved in cell-cell adhesion
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
neuron migration
protein phosphorylation
positive regulation of neuron projection development
Wnt signaling pathway
establishment of cell polarity
intracellular signal transduction
establishment or maintenance of epithelial cell apical/basal polarity
protein autophosphorylation
regulation of axonogenesis
regulation of cytoskeleton organization
multicellular organism development
phosphorylation
cell differentiation
microtubule cytoskeleton organization
mitophagy
peptidyl-threonine phosphorylation
activation of protein kinase activity
mitochondrion localization
cell-cell adhesion
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