Invitrogen

Phospho-MDM2 (Ser166) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

44-1400G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Chemically synthesized phosphopeptide derived from the region of human MDM2 that contains serine 166.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533600

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HGNC:6973; human homolog of; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2

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Gene Aliases: 1700007J15Rik; AA415488; ACTFS; hdm2; HDMX; Mdm-2; MDM2

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UniProt ID: (Human) Q00987, (Mouse) P23804

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Entrez Gene ID: (Human) 4193, (Mouse) 17246

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Function(s)
p53 binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity SUMO transferase activity enzyme binding ubiquitin protein ligase binding identical protein binding peroxisome proliferator activated receptor binding ubiquitin protein ligase activity scaffold protein binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter blood vessel development blood vessel remodeling regulation of heart rate atrioventricular valve morphogenesis endocardial cushion morphogenesis ventricular septum development atrial septum development protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest traversing start control point of mitotic cell cycle positive regulation of cell proliferation response to toxic substance response to carbohydrate response to iron ion positive regulation of gene expression negative regulation of protein processing viral process protein ubiquitination protein sumoylation peptidyl-lysine modification protein destabilization response to magnesium ion positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein localization to nucleus regulation of protein catabolic process response to cocaine response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to morphine negative regulation of DNA damage response, signal transduction by p53 class mediator establishment of protein localization response to ether negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle response to antibiotic positive regulation of protein export from nucleus response to steroid hormone cardiac septum morphogenesis cellular response to hydrogen peroxide negative regulation of cell cycle arrest cellular response to antibiotic cellular response to vitamin B1 cellular response to alkaloid cellular response to growth factor stimulus cellular response to peptide hormone stimulus cellular response to estrogen stimulus cellular response to hypoxia cellular response to UV-C regulation of signal transduction by p53 class mediator negative regulation of signal transduction by p53 class mediator negative regulation of gene expression protein catabolic process negative regulation of apoptotic process positive regulation of cell cycle
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