Invitrogen

Phospho-MEK1/MEK2 (Ser217, Ser221) Monoclonal Antibody (C.158.9)

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1 Published Figure
1 Reference
View all (33) MEK1/2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15016

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.158.9

Immunogen

Synthetic phosphopeptide corresponding to residues around pSer217/221 of human MEK1/2
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

26 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977854

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; EC 2.7.12.2; ERK activator kinase 1; ERK activator kinase 2; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK-1; MEK-2; MEK2; mitogen activated protein kinase kinase 1; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2, p45; MP2K1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen activated, kinase 2, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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Gene Aliases: AA589381; CFC3; CFC4; MAP2K1; MAP2K2; MAPKK1; MAPKK2; MEK1; MEK2; MEKK1; MK2; MKK1; MKK2; PRKMK1; PRKMK2

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UniProt ID: (Human) Q02750, (Human) P36507, (Mouse) P31938, (Rat) Q01986, (Rat) P36506, (Mouse) Q63932

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Entrez Gene ID: (Human) 5604, (Human) 5605, (Mouse) 26395, (Rat) 170851, (Rat) 58960, (Mouse) 26396

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Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine phosphatase activity protein binding ATP binding protein C-terminus binding protein kinase binding protein serine/threonine kinase activator activity protein N-terminus binding PDZ domain binding protein complex scaffold metal ion binding scaffold protein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding mitogen-activated protein kinase kinase kinase binding
Process(es)
MAPK cascade activation of MAPK activity regulation of protein phosphorylation regulation of vascular smooth muscle contraction protein phosphorylation response to oxidative stress cell cycle arrest mitotic nuclear division cell proliferation negative regulation of cell proliferation positive regulation of gene expression phosphorylation neuron differentiation keratinocyte differentiation positive regulation of cell migration melanosome transport regulation of stress-activated MAPK cascade positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of homotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation positive regulation of GTPase activity positive regulation of cell differentiation positive regulation of Ras protein signal transduction vesicle transport along microtubule Golgi inheritance response to axon injury regulation of axon regeneration neuron projection morphogenesis cell motility positive regulation of axonogenesis protein heterooligomerization response to glucocorticoid lung morphogenesis trachea formation epithelial cell proliferation involved in lung morphogenesis placenta blood vessel development labyrinthine layer development ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance cellular senescence mitophagy in response to mitochondrial depolarization regulation of early endosome to late endosome transport peptidyl-tyrosine phosphorylation peptidyl-serine autophosphorylation positive regulation of cell motility intracellular signal transduction heart development negative regulation of gene expression thyroid gland development proteolysis in other organism positive regulation of transcription, DNA-templated thymus development face development positive regulation of ERK1 and ERK2 cascade positive regulation of production of miRNAs involved in gene silencing by miRNA movement of cell or subcellular component chemotaxis signal transduction cerebellar cortex formation Bergmann glial cell differentiation
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