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Invitrogen
Phospho-MEK3/MEK6 (Ser189, Thr193, Ser207, Thr211) Polyclonal Antibody
FIGURE: 1 / 3
Phospho-MEK3/MEK6 (Ser189, Thr193, Ser207, Thr211) Antibody (44470G) in WB
Mal-E-tagged fusion protein expressing MEK6 left inactivated (6) or activated with MEKK (1-5) was spiked into background extracts and resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with the MEK3/6 (pSpT189/193)/ (pSpT207/211) antibody for two hours at room temperature in a 3% BSA-TBST buffer, followi... View More
Product Details
44470G
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK3 that contains serine 189 and threonine 193.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533659
Target Information
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases, act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 3; MAP kinase kinase 6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MEK 3; MEK 6; OTTHUMP00000166044; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPK kinase 3; Stress-activated protein kinase kinase 2; Stress-activated protein kinase kinase 3
Gene Aliases: MAP2K3; MAP2K6; MAPKK3; MAPKK6; MEK3; MEK6; MKK3; MKK6; PRKMK3; PRKMK6; SAPKK-2; SAPKK-3; SAPKK2; SAPKK3; SKK2; SKK3
UniProt ID: (Human) P46734, (Human) P52564
Entrez Gene ID: (Human) 5606, (Human) 5608
MAPK cascade
activation of MAPK activity
inflammatory response
signal transduction
peptidyl-tyrosine phosphorylation
proteolysis in other organism
regulation of cytokine biosynthetic process
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
cardiac muscle contraction
response to ischemia
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
DNA damage induced protein phosphorylation
cell cycle arrest
ovulation cycle process
positive regulation of prostaglandin secretion
response to drug
positive regulation of apoptotic process
positive regulation of nitric-oxide synthase biosynthetic process
nucleotide-binding oligomerization domain containing signaling pathway
cellular response to sorbitol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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