Invitrogen

Phospho-MEKK1 (Thr1400) Polyclonal Antibody

View all (15) MEKK1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-103271

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MAP3K1(Accession Q13233), corresponding to amino acid residues around phosphorylated Thr1400.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815970

Product Specific Information

Antibody detects endogenous levels of MEKK1 only when phosphorylated at Thr1400, which site historically referenced as Thr1402.

Target Information

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases. It is also a highly efficient activator of the JNK cascade. The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; mitogen activated protein kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

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Gene Aliases: MAP3K1; MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6

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UniProt ID: (Human) Q13233, (Rat) Q62925

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Entrez Gene ID: (Human) 4214, (Mouse) 26401, (Rat) 116667

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Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding zinc ion binding protein kinase binding nucleotide binding JUN kinase kinase kinase activity ubiquitin-protein transferase activity JUN kinase binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups mitogen-activated protein kinase kinase binding sphingolipid binding metal ion binding mitogen-activated protein kinase binding JUN kinase kinase activity
Process(es)
MAPK cascade activation of MAPKK activity MyD88-dependent toll-like receptor signaling pathway protein phosphorylation apoptotic process Fc-epsilon receptor signaling pathway cellular response to mechanical stimulus epithelial cell morphogenesis transforming growth factor beta receptor signaling pathway JNK cascade activation of JNKK activity activation of JUN kinase activity regulation of mitotic cell cycle apoptotic mitochondrial changes phosphorylation protein ubiquitination regulation of cell migration positive regulation of actin filament polymerization wound healing regulation of apoptotic process camera-type eye development eyelid development in camera-type eye protein polyubiquitination response to osmotic stress peptidyl-serine phosphorylation negative regulation of actin filament bundle assembly protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of JUN kinase activity positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of viral transcription protein oligomerization
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