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Invitrogen
Phospho-MERTK (Tyr749, Tyr753, Tyr754) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-MERTK (Tyr749, Tyr753, Tyr754) Antibody (PA5-143631) in WB
Western blot of Phospho-MERTK (Tyr749, Tyr753, Tyr754) in HEK293 lysate showing specific immunolabeling at ~160 kDa for Phospho-MERTK (Tyr749, Tyr753, Tyr754) polyclonal antibody (Product # PA5-143631) in the first lane (-). Phospho specificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 60 minutes).
Product Details
PA5-143631
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr749/753/754 of human MerTK, conjugated to keyhole limpet hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 150mM NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942860
Product Specific Information
Specific for the ~160 kDa MerTK protein phosphorylated at Tyr749/753/754. The immunolabeling is completely eliminated by treatment with lambda-phosphatase. Due to post-translational modifications of the Mer protein, a significant shift in molecular weight is seen from the predicted molecular weight of 110 kDa. For optimal results immunoprecipitation is recommended due to the 91% homology of the related receptor tyrosine kinase, Axl, that runs at ~140 kDa.
Target Information
MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-mer proto-oncogene tyrosine kinase; MER receptor tyrosine kinase; MGC133349; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase MER; Receptor tyrosine kinase MerTK; Receptor tyrosine tinase gene probably the gene for Rdy; retinal dystrophy; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyro 12; Tyrosine-protein kinase Mer
Gene Aliases: c-Eyk; c-mer; Eyk; MER; MERTK; nmf12; Nyk; rdy; RP38; Tyro12
UniProt ID: (Human) Q12866, (Mouse) Q60805, (Rat) P57097
Entrez Gene ID: (Human) 10461, (Mouse) 17289, (Rat) 65037
natural killer cell differentiation
protein phosphorylation
phagocytosis
spermatogenesis
phosphorylation
platelet activation
secretion by cell
substrate adhesion-dependent cell spreading
apoptotic cell clearance
protein kinase B signaling
positive regulation of phagocytosis
negative regulation of lymphocyte activation
retina development in camera-type eye
vagina development
negative regulation of leukocyte apoptotic process
peptidyl-tyrosine phosphorylation
cell surface receptor signaling pathway
cell-cell signaling
leukocyte migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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