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Invitrogen
Phospho-MKK7 (Ser271, Thr275) Recombinant Rabbit Monoclonal Antibody (MKK7S271T275-R4F9)
FIGURE: 1 / 3
Phospho-MKK7 (Ser271, Thr275) Antibody (MA5-37005) in WB
Western blot analysis of Phospho-MKK7 (Ser271, Thr275) using THP1 cell extract. Samples were untreated or treated with sorbitol, and incubated in monoclonal Phospho-MKK7 (Ser271, Thr275) antibody (Product # MA5-37005) with a dilution of 0.1 µg/mL.
Product Details
MA5-37005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
MKK7S271T275-R4F9
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser271/Thr275 of human phospho MKK7.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896940
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Jun N-terminal kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; JN; JNK kinase 2; JNK-activating kinase 2; JNKK 2; MAP kinase kinase 7; MAP2K4; map2k7; map2k7 {ECO:0000312|EMBL:AAX61178.1}; MAPK/ERK kinase 4; MAPK/ERK kinase 7; MAPKK 4; MAPKK 7; MEK 4; MEK 7; mitogen activated protein kinase kinase 7; protein kinase, mitogen activated, kinase 7; SAPK kinase 1; SAPK kinase 4; SAPK/ERK kinase 1; SAPKK-1; SAPKK1; SEK1; Stress-activated protein kinase kinase 4
Gene Aliases: JNKK; 2; 5930412N11Rik; JNKK 2; JNKK2; MAP2K7; MAPKK 7; MAPKK7; MEK; MEK 7; MEK7; MKK7; PRKMK7; SAPKK-4; SAPKK4; sek2; SKK4
UniProt ID: (Human) O14733, (Rat) Q4KSH7, (Mouse) Q8CE90
Entrez Gene ID: (Human) 5609, (Rat) 363855, (Mouse) 26400
magnesium ion binding
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
protein C-terminus binding
JUN kinase kinase activity
enzyme binding
protein kinase binding
protein phosphatase binding
mitogen-activated protein kinase kinase kinase binding
protein serine/threonine kinase activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
positive regulation of protein phosphorylation
protein phosphorylation
apoptotic process
response to osmotic stress
JNK cascade
activation of JUN kinase activity
response to heat
response to UV
peptidyl-tyrosine phosphorylation
response to tumor necrosis factor
positive regulation of neuron apoptotic process
stress-activated MAPK cascade
cellular response to sorbitol
signal transduction
positive regulation of telomere maintenance via telomerase
proteolysis in other organism
Fc-epsilon receptor signaling pathway
positive regulation of telomerase activity
positive regulation of telomere capping
MAPK cascade
response to stress
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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