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Invitrogen
Phospho-MKNK2 (Thr249) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-MKNK2 (Thr249) Antibody (PA5-105902) in ICC/IF
Immunocytochemistry analysis of Phospho-MKNK2 (Thr249) in Hela cells (Forskolin 500 μm for 30 min). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-MKNK2 (Thr249) polyclonal antibody (Product # PA5-105902) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti... View More
Product Details
PA5-105902
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MKNK2(Accession Q9HBH9), corresponding to amino acid residues around phosphorylated Thr249.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2817301
Product Specific Information
Antibody detects endogenous levels of MKNK2 only when phosphorylated at Thr249.
Target Information
The Map kinase-interacting kinases (MKNK1 and MKNK2) are activated by Map kinases, regulate protein synthesis by phosphroylation eIF4E on Ser209, and may contribute to tumorigenesis. Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G protein-coupled receptor kinase 7; MAP kinase signal-integrating kinase 2; MAP kinase-interacting serine/threonine-protein kinase 2; MAPK signal-integrating kinase 2; mnk2
Gene Aliases: 2010016G11Rik; GPRK7; MKNK2; MNK2
UniProt ID: (Human) Q9HBH9, (Mouse) Q8CDB0, (Rat) Q5U2N4
Entrez Gene ID: (Human) 2872, (Mouse) 17347, (Rat) 299618
regulation of translation
protein phosphorylation
cell surface receptor signaling pathway
peptidyl-serine phosphorylation
hemopoiesis
intracellular signal transduction
protein autophosphorylation
cellular response to arsenic-containing substance
extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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