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Phospho-MKP1/MKP2 (Ser296, Ser318) Antibody (PA5-104854) in ICC/IF
Immunofluorescent analysis of Phospho-MKP1/MKP2 (Ser296, Ser318) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MKP1/MKP2 (Ser296, Ser318) polyclonal antibody (Product # PA5-104854) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-r... View More
Product Details
PA5-104854
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DUSP1(Accession P28562), corresponding to amino acid residues around phosphorylated Ser296.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816327
Product Specific Information
Antibody detects endogenous levels of MKP1/2 only when phosphorylated at Serine 296/318.
Target Information
There are ten mitogen-activated protein kinase (MAPK) phosphatases (MKPs) that act as negative regulators of MAPK activity in mammalian cells. MKP2 (DUSP4) is a dual threonine/tyrosine phosphatase that specifically dephosphorylates and inactivates MAPKs (ERK, JNK, p38). It contains one Rhodanese domain and one tyrosine-protein phosphatase domain. MKP2 is expressed in a variety of tissues, and is localized in the nucleus. Transcription factor E2F-1 acts as a transcriptional regulator of MKP2. E2F-1/MKP2 pathway mediates apoptosis under oxidative stress and that MKP2 suppresses tumor formation. MKP2 is a novel transcription target of p53 in mediating apoptosis by 10-bp palindrome motif (CTGGCGCCAG) in the MKP2 promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3CH134/CL100 PTPase; CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH1; Dual specificity protein phosphatase hVH2; DUSP 4; DUSP-4; MAP kinase phosphatase 1; MAP kinase phosphatase 2; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; Mitogen-activated protein kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 2; mitogen-activated protein kinase phosphatase-1; MKP II; MKPII; oxidative stress-inducible protein tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 16; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; Protein-tyrosine phosphatase non-receptor type 16; serine/threonine specific protein phosphatase; VH1 homologous phosphatase 2
Gene Aliases: 2700078F24Rik; 3CH134; AI844617; BB104621; CL100; DUSP1; DUSP4; E130306H24Rik; erp; HVH1; HVH2; MKP-1; MKP-2; MKP1; MKP2; PTPN10; Ptpn16; TYP; U19515; VH1; VH2
UniProt ID: (Human) P28562, (Human) Q13115, (Rat) Q64623, (Mouse) P28563, (Rat) Q62767, (Mouse) Q8BFV3
Entrez Gene ID: (Human) 1843, (Human) 1846, (Rat) 114856, (Mouse) 19252, (Rat) 60587, (Mouse) 319520
non-membrane spanning protein tyrosine phosphatase activity
protein binding
protein tyrosine/threonine phosphatase activity
MAP kinase tyrosine/serine/threonine phosphatase activity
growth factor binding
protein tyrosine phosphatase activity
phosphatase activity
MAP kinase threonine phosphatase activity
protein tyrosine/serine/threonine phosphatase activity
phosphoprotein phosphatase activity
hydrolase activity
inactivation of MAPK activity
endoderm formation
dephosphorylation
peptidyl-tyrosine dephosphorylation
peptidyl-threonine dephosphorylation
negative regulation of ERK1 and ERK2 cascade
protein dephosphorylation
response to light stimulus
response to organic substance
response to estradiol
response to retinoic acid
cellular response to hormone stimulus
response to testosterone
intracellular signal transduction
response to hydrogen peroxide
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
response to glucocorticoid
negative regulation of meiotic cell cycle
response to cAMP
response to calcium ion
mitotic cell cycle arrest
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of DNA biosynthetic process
response to oxidative stress
regulation of apoptotic process
cell cycle
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