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Invitrogen
Phospho-MLKL (Ser345) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-MLKL (Ser345) Antibody (PA5-121360) in WB
Western blot analysis of Phospho-MLKL (Ser345) in L929 cells. Samples were incubated with Phospho-MLKL (Ser345) Polyclonal antibody (Product # PA5-121360) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. L929 cells were treated by 20 ng/mL TNF alpha 100 nM Smac mimetic and 20 μM z-VAD for 8h. Lysates/proteins: 25 µg per lane. Blocking buffer... View More
Product Details
PA5-121360
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S345 of mouse MLKL (NP_001297542.1).
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914932
Product Specific Information
Positive test controls include: HeLa. The target is usually found in the following locations: Cell membrane, Cytoplasm.
Immunogen sequence: TQNSIS
Target Information
The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mixed lineage kinase domain-like protein; mlkl
Gene Aliases: 9130019I15Rik; Mlkl
UniProt ID: (Mouse) Q9D2Y4
Entrez Gene ID: (Mouse) 74568
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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