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Invitrogen
Phospho-MYH9 (Ser1803) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-MYH9 (Ser1803) Antibody (PA5-143718) in WB
Western blot of Phospho-MYH9 (Ser1803) in human A431 cells stimulated with calyculin A (100 nM, 30 minutes). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit Myosin IIA Heavy Chain (a.a. 1936-1950) polyclonal (lanes 1 & 2), Phospho-MYH9 (Ser1803) polyclonal antibody (Product # PA5-143718) (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) ... View More
Product Details
PA5-143718
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Myosin IIA Heavy Chain (Ser1803) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser1803 in human myosin IIA heavy chain. This sequence is highly conserved in rat and mouse myosin IIA heavy chain and some homology to conserved sites in non-muscle myosin IIB and smooth muscle myosin heavy chains.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942946
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated Myosin IIA Heavy Chain (Ser-1803) peptide before affinity purification using phospho-Myosin IIA Heavy Chain (Ser-1803) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to Myosin IIA Heavy Chain on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A. This reactivity is removed after treatment with lambda phosphatase.
Target Information
TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular myosin heavy chain, type A; flectin; MGC104539; Myosin heavy chain 9; myosin heavy chain IX; Myosin heavy chain, non-muscle IIa; myosin IIA; Myosin-9; myosin-9-like; myosin9; NMHC II-A; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
Gene Aliases: BDPLT6; DFNA17; EPSTS; Fltn; FTNS; MHA; MYH9; Myhn-1; Myhn1; NMHC-II-A; NMHCIIA; NMMHC-A; NMMHC-IIA; NMMHCA; TU72.6
UniProt ID: (Human) P35579, (Mouse) Q8VDD5
Entrez Gene ID: (Rat) 100911597, (Human) 4627, (Mouse) 17886
microfilament motor activity
motor activity
actin binding
ATP binding
ATPase activity
actin-dependent ATPase activity
protein homodimerization activity
protein anchor
ADP binding
poly(A) RNA binding
actin filament binding
protein binding
calmodulin binding
protein domain specific binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
meiotic spindle organization
cell morphogenesis involved in differentiation
cytokinesis
angiogenesis
in utero embryonic development
establishment of T cell polarity
membrane protein ectodomain proteolysis
phagocytosis, engulfment
movement of cell or subcellular component
cell adhesion
myoblast fusion
regulation of cell shape
protein transport
single organismal cell-cell adhesion
actin filament polymerization
actin filament-based movement
platelet formation
actomyosin structure organization
actin cytoskeleton reorganization
uropod organization
blood vessel endothelial cell migration
establishment of meiotic spindle localization
actin filament capping
platelet aggregation
negative regulation of actin filament severing
positive regulation of protein processing in phagocytic vesicle
integrin-mediated signaling pathway
monocyte differentiation
leukocyte migration
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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