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Phospho-MYL12A (Ser1) Antibody (PA5-143726) in ICC/IF
Immunocytochemistry analysis of Phospho-MYL12A (Ser1) in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (middle) and MYL9/MYL12 polyclonal antibody (Product # PA5-143725) (top left), Phospho-MYL12A (Ser19) polyclonal antibody (Product # PA5-143727) (middle left) and Phospho-MYL12A (Ser1) polyclonal antibody (Product # PA5-143726) (bottom left). Goat anti-Mouse DyLight® 488 and ... View More
Product Details
PA5-143726
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-MLC (Ser-1) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1 in bovine myosin regulatory light chain 9 (Myl9). This peptide sequence is highly conserved in human, rat, and mouse Myl9, and in other smooth muscle and non-muscle MLCs.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942954
Product Specific Information
The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF or calyculin A. In addition, the antibody detects the 20 kDa myosin light chain in mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.
Target Information
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain specific myosin regulatory light chain; Epididymis secretory protein Li 24; fast skeletal myosin light chain 2; HEL-S-24; MLC-2; MLC-2A; MLC-2B; MLC20; myosin light chain, regulatory B-like; Myosin regulatory light chain 12A; Myosin regulatory light chain 12B; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 2-A, smooth muscle isoform; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; myosin regulatory light chain 3; Myosin regulatory light chain 9; myosin regulatory light chain MRCL3; Myosin regulatory light chain MRLC2; Myosin regulatory light chain MRLC3; Myosin regulatory light chain RLC-A; Myosin regulatory light polypeptide 9; Myosin RLC; Myosin RLC-A; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); NMDA receptor-interacting protein; SHUJUN-1
Gene Aliases: 2900073G15Rik; AI838292; BOS_22276; C80200; HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC2; MRLC3; MYL12A; MYL12B; MYL2B; MYL9; MYLC2B; MYRL2; RGD1309537; RLC; Rlc-a
UniProt ID: (Human) P19105, (Bovine) Q5E9E2, (Rat) P13832
Entrez Gene ID: (Human) 10627, (Bovine) 531505, (Rat) 501203, (Mouse) 67268
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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