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Phospho-MYL12A (Ser19) Antibody (PA5-143727) in ICC/IF
Immunocytochemistry analysis of Phospho-MYL12A (Ser19) in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (middle) and MYL9/MYL12 polyclonal antibody (Product # PA5-143725) (top left), Phospho-MYL12A (Ser19) polyclonal antibody (Product # PA5-143727) (middle left) and Phospho-MYL12A (Ser1) polyclonal antibody (Product # PA5-143726) (bottom left). Goat anti-Mouse DyLight® 488 and... View More
Product Details
PA5-143727
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-MLC (Ser-19) synthetic peptide (coupled to KLH) corresponds to amino acid residues surrounding Ser-19 in human myosin regulatory light chain 12A (Myl12A). This peptide sequence is highly conserved in human, rat, mouse, chicken, and zebrafish Myl12A, and in other smooth muscle and non-muscle MLCs.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942955
Product Specific Information
This antibody was cross-adsorbed to MLC (a.a. 11-22) then affinity purified using phospho-MLC (Ser-19) peptide (without carrier). The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF and in mouse C2C12 treated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment of MLC.
Target Information
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain specific myosin regulatory light chain; Epididymis secretory protein Li 24; HEL-S-24; MLC-2; MLC-2A; MLC-2B; MLC20; myosin light chain, regulatory B-like; Myosin regulatory light chain 12A; Myosin regulatory light chain 12B; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain 2-A, smooth muscle isoform; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; myosin regulatory light chain 3; Myosin regulatory light chain MRLC2; Myosin regulatory light chain MRLC3; Myosin regulatory light chain RLC-A; Myosin RLC; Myosin RLC-A; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); NMDA receptor-interacting protein; SHUJUN-1
Gene Aliases: 2900073G15Rik; AI838292; C80200; HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC2; MRLC3; MYL12A; MYL12B; MYL2B; MYLC2B; RGD1309537; RLC; Rlc-a
UniProt ID: (Human) P19105, (Rat) P13832
Entrez Gene ID: (Human) 10627, (Rat) 501203, (Mouse) 67268
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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