Invitrogen

Phospho-MYL9 (Thr18, Ser19) Polyclonal Antibody

4 Published Figures

11 References

View all (22) MYL9 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

Phospho-MYL9 (Thr18, Ser19) Antibody in Western Blot (WB)

FIGURE: 1 / 5

Phospho-MYL9 (Thr18, Ser19) Antibody (PA5-17727) in WB

Western blot analysis of extracts from HEK293 cells stimulated with calcium ionophore A23187 for the indicated times, using Phospho-MYL9 (Thr18/Ser19) Antibody (Product # PA5-17727).

Phospho-MYL9 (Thr18, Ser19) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

PA5-17727

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr18/Ser19 of human myosin light chain 2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

20 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10980950

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the cardiac isoform of myosin light chain 2.

Target Information

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin light chain may regulate muscle contraction by modulating the ATPase activity of myosin heads. It also binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Formin Activity and mDia1 Contribute to Maintain Axon Initial Segment Composition and Structure.

Molecular neurobiology

Zhang W,Ciorraga M,Mendez P,Retana D,Boumedine-Guignon N,Achón B,Russier M,Debanne D,Garrido JJ

Published figure using Phospho-MYL9 (Thr18, Ser19) polyclonal antibody (Product # PA5-17727) in Immunocytochemistry

Wed Dec 01 00:00:00 UTC 2021

Factor quinolinone inhibitors alter cell morphology and motility by destabilizing interphase microtubules.

Scientific reports

Stoiber P,Scribani Rossi P,Pokharel N,Germany JL,York EA,Schaus SE,Hansen U

PA5-17727 was used in Western Blot to show that FQI1 interferes with microtubule-associated functions in interphase, specifically cell morphology and motility.

Tue Dec 07 00:00:00 UTC 2021

ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells.

eLife

Ullo MF,Logue JS

PA5-17727 was used in Immunocytochemistry to identify cofilin-1 and ADF as actin remodeling factors required for fast amoeboid migration.

Fri Jun 25 00:00:00 UTC 2021

Endothelial cell-derived SSAO can increase MLC₂₀ phosphorylation in VSMCs.

Open life sciences

Zhang Y,Zhang X,Cao Z,Huang Y,Zheng Y,Yang X

PA5-17727 was used in Western Blotting to find that the increased SSAO by AOC3 overexpression can decrease the iNOS expression level and its activity after hypoxia.

Tue Nov 02 00:00:00 UTC 2021

17‑AAG synergizes with Belinostat to exhibit a negative effect on the proliferation and invasion of MDA‑MB‑231 breast cancer cells.

Oncology reports

Zuo Y,Xu H,Chen Z,Xiong F,Zhang B,Chen K,Jiang H,Luo C,Zhang H

PA5-17727 was used in Western Blotting to provide a beneficial reference for the clinical treatment of patients with TNBC.

Mon Jun 01 00:00:00 UTC 2020

Histone deacetylase inhibitor CG200745 ameliorates high-fat diet-induced hypertension via inhibition of angiotensin II production.

Naunyn-Schmiedeberg's archives of pharmacology

Yoon GE,Jung JK,Lee YH,Jang BC,In Kim J

PA5-17727 was used in Western Blotting to investigate the role and underlying mechanism of CG200745 in high-fat diet (HFD)-induced hypertension.

Sun Mar 01 00:00:00 UTC 2020

Histone deacetylase inhibitor CG200745 ameliorates high-fat diet-induced hypertension via inhibition of angiotensin II production.

Naunyn-Schmiedeberg's archives of pharmacology

Yoon GE,Jung JK,Lee YH,Jang BC,In Kim J

PA5-17727 was used in Western Blotting to investigate the role and underlying mechanism of CG200745 in high-fat diet (HFD)-induced hypertension.

Sun Mar 01 00:00:00 UTC 2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl₂.

Scientific reports

Magdaleno C,Dixon L,Rajasekaran N,Varadaraj A

PA5-17727 was used in Western Blotting to systematically reexamine the role of hypoxia mimetics as experimental substitutes for hypoxia and provides new findings on HIFα stabilization and the FN matrix in the context of renal cancer.

Thu Oct 29 00:00:00 UTC 2020

A two-phase response of endothelial cells to hydrostatic pressure.

Journal of cell science

Prystopiuk V,Fels B,Simon CS,Liashkovich I,Pasrednik D,Kronlage C,Wedlich-Söldner R,Oberleithner H,Fels J

PA5-17727 was used in Western Blotting to characterise the specific response of endothelial cells to application of pressure by using a combination of quantitative fluorescence microscopy, atomic force microscopy and molecular perturbations.

Thu Jun 21 00:00:00 UTC 2018

Rnd3 regulates lung cancer cell proliferation through notch signaling.

PloS one

Tang Y,Hu C,Yang H,Cao L,Li Y,Deng P,Huang L

PA5-17727 was used in western blot to explore the role of Rnd3 in non-small cell lung cancer

Thu Jul 09 00:00:00 UTC 2015

Rnd3 haploinsufficient mice are predisposed to hemodynamic stress and develop apoptotic cardiomyopathy with heart failure.

Cell death & disease

Yue X,Yang X,Lin X,Yang T,Yi X,Dai Y,Guo J,Li T,Shi J,Wei L,Fan GC,Chen C,Chang J

PA5-17727 was used in western blot to study heart failure in Rnd3(+/-) haploinsufficient mice

Thu Jun 05 00:00:00 UTC 2014

New insights into myosin phosphorylation during cyclic nucleotide-mediated smooth muscle relaxation.

Journal of muscle research and cell motility

Puetz S,Schroeter MM,Piechura H,Reimann L,Hunger MS,Lubomirov LT,Metzler D,Warscheid B,Pfitzer G

PA5-17727 was used in western blot to study the effects of cyclic nucleotide-mediated smooth muscle relaxation on the phosphorylation patterns of myosin regulatory light chains

Sat Dec 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 20 kDa myosin light chain; LC20; MGC3505; MLC-2C; myosin light chain, regulatory C; myosin regulatory light chain; myosin regulatory light chain 1; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory

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Gene Aliases: AI327049; LC20; MLC-2C; MLC2; MLC20; MRLC1; MYL9; Mylc2c; MYRL2; RLC-C

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UniProt ID: (Human) P24844, (Mouse) Q9CQ19

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Entrez Gene ID: (Human) 10398, (Mouse) 98932

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Function(s)

calcium ion binding structural constituent of muscle myosin heavy chain binding metal ion binding

Process(es)

platelet aggregation muscle contraction regulation of muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-MYL9 (Thr18, Ser19) Polyclonal Antibody

Phospho-MYL9 (Thr18, Ser19) Antibody (PA5-17727) in WB

Product Details

PA5-17727

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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