Invitrogen

Phospho-MYLK (Ser1760) Polyclonal Antibody

3 Published Figures

5 References

View all (27) MYLK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

Phospho-MYLK (Ser1760) Antibody in Western Blot (WB)

FIGURE: 1 / 4

Phospho-MYLK (Ser1760) Antibody (44-1085G) in WB

Lysates prepared from COS cells transfected with muscle MLCK left unstimulated, or phosphorylated in vitro by PKA, were resolved by SDS-PAGE on a 4-12% polyacrylamide gel and transferred to PVDF. Membranes were left untreated or treated with lambda phophatase, blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with MLCK (pS1760) antibody for two hours at room temperature in 3%... View More

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Product Details

44-1085G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rabbit

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human myosin light chain kinase (MLCK) that contains serine 1760. The sequence is conserved in human, mouse and rat.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533570

Target Information

MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting NOX4 alleviates sepsis-induced acute lung injury via attenuation of redox-sensitive activation of CaMKII/ERK1/2/MLCK and endothelial cell barrier dysfunction.

Redox biology

Jiang J,Huang K,Xu S,Garcia JGN,Wang C,Cai H

44-1085G was used in Western Blotting to identify a novel, selective and causal role of NOX4 (versus other NOX isoforms) in inducing lung EC barrier dysfunction and injury/mortality in a preclinical CLP-induced septic model, which involves redox-sensitive activation of CaMKII/ERK1/2/MLCK pathway. Targeting NOX4 may therefore prove to an innovative therapeutic option that is markedly effective in treating ALI/ARDS.

Tue Sep 01 00:00:00 UTC 2020

Targeting NOX4 alleviates sepsis-induced acute lung injury via attenuation of redox-sensitive activation of CaMKII/ERK1/2/MLCK and endothelial cell barrier dysfunction.

Redox biology

Jiang J,Huang K,Xu S,Garcia JGN,Wang C,Cai H

Tue Sep 01 00:00:00 UTC 2020

Selexipag Active Metabolite ACT-333679 Displays Strong Anticontractile and Antiremodeling Effects but Low β-Arrestin Recruitment and Desensitization Potential.

The Journal of pharmacology and experimental therapeutics

Gatfield J,Menyhart K,Wanner D,Gnerre C,Monnier L,Morrison K,Hess P,Iglarz M,Clozel M,Nayler O

Published figure using Phospho-MYLK (Ser1760) polyclonal antibody (Product # 44-1085G) in Western Blot

Sat Jul 01 00:00:00 UTC 2017

Phosphorylation of proteins during human myometrial contractions: A phosphoproteomic approach.

Biochemical and biophysical research communications

Hudson CA,López Bernal A

441085G was used in western blot to examine phosphorylated proteins during spontaneous and oxytocin-driven phasic contractions

Sun Jan 22 00:00:00 UTC 2017

Role of CaMKII in Ang-II-dependent small artery remodeling.

Vascular pharmacology

Prasad AM,Ketsawatsomkron P,Nuno DW,Koval OM,Dibbern ME,Venema AN,Sigmund CD,Lamping KG,Grumbach IM

441085G was used in western blot to discover that calcium-calmodulin-dependent kinase II is an important regulator of smooth muscle function in angiotensin-II hypertension

Thu Dec 01 00:00:00 UTC 2016

Steroid receptor co-activator interacting protein (SIP) mediates EGF-stimulated expression of the prostaglandin synthase COX2 and prostaglandin release in human myometrium.

Molecular human reproduction

Hudson CA,McArdle CA,López Bernal A

44-1085G was used in immunoprecipitation and western blot to assess the mediation of EGF-stimulated expression of the prostaglandin synthase COX2 and prostaglandin release in human myometrium via the steroid receptor co-activator interacting protein (SIP

Fri Jul 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Kinase-related protein; KRP; MLCK; mylk; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin

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Gene Aliases: 9530072E15Rik; A930019C19Rik; AAT7; AW489456; BOS_827; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK1; nmMlck; smMLCK

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UniProt ID: (Human) Q15746, (Bovine) Q28824

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Entrez Gene ID: (Human) 4638, (Rabbit) 100009143, (Bovine) 338037, (Mouse) 107589

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Function(s)

actin binding myosin light chain kinase activity protein binding calmodulin binding ATP binding metal ion binding nucleotide binding protein kinase activity protein serine/threonine kinase activity calmodulin-dependent protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding

Process(es)

positive regulation of defense response to virus by host protein phosphorylation smooth muscle contraction actin filament organization tonic smooth muscle contraction phosphorylation positive regulation of cell migration bleb assembly positive regulation of calcium ion transport aorta smooth muscle tissue morphogenesis cellular hypotonic response positive regulation of wound healing mitophagy in response to mitochondrial depolarization muscle contraction xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-MYLK (Ser1760) Polyclonal Antibody

Phospho-MYLK (Ser1760) Antibody (44-1085G) in WB

Product Details

44-1085G

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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