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Invitrogen
Phospho-MYPT1 (Ser472) Polyclonal Antibody
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Phospho-MYPT1 (Ser472) Antibody (PA5-114608) in WB
Western blot analysis of Phospho-MYPT1 (Ser472) in HeLa whole cells lysates (absence (-) or presence (+) of N peptide (non-phospho peptide) and P (phospho peptide)). Samples were incubated with polyclonal antibody (Product # PA5-114608).
Product Details
PA5-114608
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYPT1 around the phosphorylation site of Ser472.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899244
Product Specific Information
Antibody detects endogenous levels of MYPT1 only when phosphorylated at Ser472.
Target Information
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MBSP; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; Myosin phosphatase-targeting subunit 1; PP-1M; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Protein phosphatase myosin-binding subunit; Protein phosphatase subunit 1M; Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit
Gene Aliases: 1200015F06Rik; 5730577I22Rik; AA792106; AV099298; D10Ertd625e; M110; M130; MBS; MBSP; MYPT1; PPP1R12A
UniProt ID: (Human) O14974, (Rat) Q10728, (Mouse) Q9DBR7
Entrez Gene ID: (Human) 4659, (Rat) 116670, (Mouse) 17931
protein dephosphorylation
mitotic nuclear division
regulation of cell adhesion
regulation of myosin-light-chain-phosphatase activity
negative regulation of catalytic activity
positive regulation of transcription from RNA polymerase II promoter
regulation of nucleocytoplasmic transport
centrosome organization
signal transduction
G2/M transition of mitotic cell cycle
positive regulation of myosin-light-chain-phosphatase activity
cellular response to drug
regulation of establishment of endothelial barrier
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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