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Phospho-Myosin (Ser19, Ser20) Antibody (100-401-416) in WB
Western Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: HeLa whole cell lysate. Lane 2: HeLa whole cell lysate + smooth muscle recombinant phospho protein. Lane 3: HeLa whole cell lysate + regulatory light chain recombinant phospho protein. Load: 10 µg of whole cell lysate + 1.0 µg of recombinant protein. Primary antibody: Myosin pS19/pS20 antibody at 1.0 µg/mL overnight at 4°C. Secondary antibody: P... View More
Product Details
100-401-416
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
70 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Target Information
Myosin regulatory light polypeptide 9 is a highly conserved, ubiquitously expressed protein that in humans is encoded by the MYL9 gene. Myosin, a structural component of muscle, are hexameric proteins consisting of two heavy chains and four light chains, including a single pair of nonphosphorylatable light chain subunits and another pair of phosphorylatable light chain subunits.The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. Myosin light chain (Myl) 9 and Myl12 are new functional ligands for CD69. Blockade of CD69-Myl9/12 interactio ameliorates allergic airway inflammation in ovalbumin-induced and house dust mite-induced mouse models of asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 20 kDa myosin light chain; Epididymis secretory protein Li 24; HEL-S-24; LC20; MLC-2; MLC-2A; MLC-2B; MLC-2C; MLC20; myosin regulatory light chain 1; Myosin regulatory light chain 12A; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; myosin regulatory light chain 3; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light chain MRLC2; Myosin regulatory light chain MRLC3; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light chain 12B, regulatory; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); SHUJUN-1
Gene Aliases: HEL-S-24; LC20; MLC-2B; MLC-2C; MLC-B; MLC2; MLCB; MRCL3; MRLC1; MRLC2; MRLC3; MYL12A; MYL12B; MYL2B; MYL9; MYLC2B; MYRL2; RLC
UniProt ID: (Human) P19105, (Human) O14950, (Human) P24844
Entrez Gene ID: (Human) 10627, (Human) 103910, (Human) 10398
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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