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Invitrogen
Phospho-NGEF (Tyr87) Polyclonal Antibody
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Phospho-NGEF (Tyr87) Antibody (PA5-143697) in ICC/IF
Immunocytochemistry analysis of Phospho-NGEF (Tyr87) in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal Ephexin-1 (C-terminal region) antibody and Phospho-NGEF (Tyr87) polyclonal antibody (Product # PA5-143697), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Product Details
PA5-143697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-ephexin-1 (Tyr-87) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 87 in mouse ephexin-1. This sequence has significant homology to the conserved region in human and rat ephexin-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942925
Product Specific Information
The antibody detects an 80 kDa* band corresponding to ephexin-1 in Western blots of human A431 treated with pervanadate. This band is removed by alkaline phosphatase treatment.
Target Information
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain-enriched SH3-domain protein Besh3; Eph-interacting exchange protein; Ephexin-1; Neuronal guanine nucleotide exchange factor; transforming immortalised mammary epithelial sequence 2
Gene Aliases: ARHGEF27; Besh3; EPHEXIN; NGEF; Tims2
UniProt ID: (Human) Q8N5V2, (Mouse) Q8CHT1
Entrez Gene ID: (Human) 25791, (Mouse) 53972, (Rat) 246217
multicellular organism development
nervous system development
cellular process
cell differentiation
regulation of Rho protein signal transduction
regulation of GTPase activity
positive regulation of GTPase activity
ephrin receptor signaling pathway
negative regulation of dendritic spine morphogenesis
positive regulation of apoptotic process
regulation of small GTPase mediated signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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