Invitrogen

Phospho-NMDAR2B (Tyr1472) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

OPA1-04116

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 of rat GRIN2B conjugated to KLH
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325370

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and zebrafish based on 100% sequence homology.

In Western blot, this antibody detects a ~180 kDa protein representing NMDAR NR2B-subunit protein phosphorylated at Tyr1472. It also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either lamda-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Target Information

NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GluN2B; GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NMDE2; NME2; NR2B

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Gene Aliases: AW490526; GluN2B; Grin2b; Nmdar2b; NR2B

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UniProt ID: (Mouse) Q01097, (Rat) Q00960

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Entrez Gene ID: (Mouse) 14812, (Rat) 24410

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Function(s)
receptor activity ionotropic glutamate receptor activity NMDA glutamate receptor activity receptor binding interleukin-1 receptor binding ion channel activity extracellular-glutamate-gated ion channel activity cation channel activity calcium channel activity protein binding beta-catenin binding drug binding zinc ion binding glycine binding D2 dopamine receptor binding ionotropic glutamate receptor binding neurotransmitter binding metal ion binding protein heterodimerization activity cell adhesion molecule binding
Process(es)
action potential behavioral fear response in utero embryonic development startle response suckling behavior transport ion transport cation transport calcium ion transport chemical synaptic transmission sensory organ development learning or memory learning memory associative learning embryo development regulation of protein kinase A signaling positive regulation of cell death positive regulation of glutamate secretion ion transmembrane transport ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic fear response receptor clustering regulation of MAPK cascade response to ethanol regulation of synaptic plasticity regulation of neuronal synaptic plasticity regulation of long-term neuronal synaptic plasticity behavioral response to pain rhythmic process positive regulation of synaptic transmission detection of mechanical stimulus involved in sensory perception of pain regulation of postsynaptic membrane potential excitatory postsynaptic potential long-term synaptic potentiation calcium ion transmembrane transport cation transmembrane transport response to amphetamine long-term memory response to mechanical stimulus response to toxic substance response to carbohydrate response to manganese ion cellular response to magnesium starvation response to organic cyclic compound response to amine hippocampus development cerebral cortex development response to magnesium ion multicellular organismal response to stress response to cytokine response to cocaine response to calcium ion response to methylmercury response to electrical stimulus response to other organism response to growth hormone response to fungicide cellular response to amino acid stimulus cellular response to manganese ion cellular response to dsRNA cellular response to growth factor stimulus cellular response to lipid cellular response to organic cyclic compound
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