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Phospho-NOTCH3 (Ser84) Antibody (PA5-105323) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-NOTCH3 (Ser84) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-NOTCH3 (Ser84) polyclonal antibody (Product # PA5-105323) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105323
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NOTCH3(Accession Q9UM47), corresponding to amino acid residues around phosphorylated Ser84.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816751
Product Specific Information
Antibody detects endogenous levels of NOTCH3 only when phosphorylated at Ser84.
Target Information
NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neurogenic locus notch homolog protein 3; Notch 3; Notch gene homolog 3; Notch homolog 3
Gene Aliases: AW229011; CADASIL; CADASIL1; CASIL; hpbk; IMF2; LMNS; N3; NOTCH3
UniProt ID: (Human) Q9UM47, (Rat) Q9R172, (Mouse) Q61982
Entrez Gene ID: (Human) 4854, (Rat) 56761, (Mouse) 18131
transcription, DNA-templated
regulation of transcription, DNA-templated
Notch signaling pathway
multicellular organism development
cell differentiation
forebrain development
tissue regeneration
negative regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of smooth muscle cell proliferation
neuron fate commitment
glomerular capillary formation
negative regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
Notch receptor processing
positive regulation of transcription from RNA polymerase II promoter
artery morphogenesis
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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