Invitrogen

Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Recombinant Rabbit Monoclonal Antibody (SU03-70)

View all (11) PAK1/2/3 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32130

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SU03-70

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser144 of Human PAK1 aa 121-150
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809421

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: activated protein kinase alpha; ADRB2; adriamycin resistance-associated; Alpha-PAK; Beta-PAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; Oligophrenin-3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK65; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast

View more View less

Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; ARA; AW045634; beta-PAK; bPAK; D16Ertd269e; gamma-PAK; mKIAA4182; MRX30; MRX47; OPHN3; PAK-1; PAK-2; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; Pak65alpha; Pak65beta; Paka; PAKalpha; Pakb; PAKgamma; Stk4

View more View less

UniProt ID: (Human) Q13153, (Human) Q13177, (Human) O75914, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) Q61036, (Rat) Q62829

View more View less

Entrez Gene ID: (Human) 5058, (Human) 5062, (Human) 5063, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433

View more View less

Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding collagen binding ATP binding protein kinase binding protein tyrosine kinase activator activity identical protein binding cadherin binding involved in cell-cell adhesion MAP kinase kinase activity Rho GTPase binding SH3 domain binding metal ion binding nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity GTPase binding
Process(es)
response to hypoxia positive regulation of protein phosphorylation protein phosphorylation exocytosis apoptotic process Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation dendrite development cell migration amygdala development signal transduction by protein phosphorylation actin cytoskeleton organization stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway wound healing regulation of apoptotic process receptor clustering regulation of MAPK cascade positive regulation of JUN kinase activity protein autophosphorylation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition dendritic spine development gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation MAPK cascade mitotic cell cycle stimulatory C-type lectin receptor signaling pathway axonogenesis positive regulation of cell proliferation positive regulation of cell migration T cell costimulation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ephrin receptor signaling pathway T cell receptor signaling pathway activation of MAPK activity multicellular organism development regulation of neuron projection development regulation of actin filament polymerization positive regulation of neuron apoptotic process positive regulation of dendritic spine morphogenesis positive regulation of DNA biosynthetic process peptidyl-serine phosphorylation regulation of growth negative regulation of apoptotic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein tyrosine kinase activity positive regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis negative regulation of protein kinase activity signal transduction viral process vascular endothelial growth factor receptor signaling pathway regulation of defense response to virus by virus cell-cell adhesion activation of MAPKK activity small GTPase mediated signal transduction intracellular signal transduction synapse organization dendritic spine morphogenesis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support