Invitrogen

Phospho-PAK1/2/3 (Thr402) Polyclonal Antibody

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View all (11) PAK1/2/3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-4636

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
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Product Specifications

Species Reactivity

Mouse, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat PAK1-3 conjugated to KLH
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2160238

Product Specific Information

This antibody is predicted to react with bovine, canine, human and mouse based on 100% sequence homology.

This antibody is specific for the ~68 kDa to ~70 kDa PAK phosphorylated at Thr402. Immunolabeling can be blocked by lambda-phosphatase treatment.

Target Information

In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: activated protein kinase alpha; ADRB2; Alpha-PAK; Beta-PAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3

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Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; AW045634; beta-PAK; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-1; PAK-2; PAK-3; Pak1; Pak2; Pak3; Pak65alpha; Pak65beta; Paka; Pakb; Stk4

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UniProt ID: (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) Q61036, (Rat) Q62829

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Entrez Gene ID: (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity protein binding collagen binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding Rac GTPase binding MAP kinase kinase kinase activity protein tyrosine kinase activator activity identical protein binding receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity Rho GTPase binding SH3 domain binding metal ion binding GTPase binding
Process(es)
response to hypoxia positive regulation of protein phosphorylation protein phosphorylation exocytosis apoptotic process Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation dendrite development cell migration amygdala development signal transduction by protein phosphorylation actin cytoskeleton organization stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway wound healing regulation of apoptotic process receptor clustering regulation of MAPK cascade positive regulation of JUN kinase activity protein autophosphorylation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition dendritic spine development gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation MAPK cascade activation of MAPK activity multicellular organism development axonogenesis regulation of neuron projection development regulation of actin filament polymerization positive regulation of neuron apoptotic process positive regulation of dendritic spine morphogenesis positive regulation of DNA biosynthetic process peptidyl-serine phosphorylation regulation of growth negative regulation of apoptotic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein tyrosine kinase activity positive regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis activation of MAPKK activity mitotic cell cycle small GTPase mediated signal transduction intracellular signal transduction
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