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Invitrogen
Phospho-PAK4/6/7 (Ser474) Recombinant Rabbit Monoclonal Antibody (1H7L3)
FIGURE: 1 / 2
Phospho-PAK4/6/7 (Ser474) Antibody (702663) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of Phospho-PAK4 + PAK6 + PAK7 pSer474 using Anti- Phospho-PAK4 + PAK6 + PAK7 pSer474 Recombinant Rabbit Monoclonal Antibody (Product # 702663, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representativ... View More
Product Details
702663
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H7L3
Immunogen
Peptide corresponding to human Phospho-PAK4/PAK5/PAK6 (Ser474) (aa472-aa480)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2688293
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Rat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK 4/5/6; PAK 4/6/7; PAK-4; PAK-5; PAK-6; PAK-7; PAK4/5/6; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; Serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK7
Gene Aliases: KIAA1142; KIAA1264; PAK4; PAK5; PAK6; PAK7
UniProt ID: (Human) O96013, (Human) Q9P286, (Human) Q9NQU5
Entrez Gene ID: (Human) 10298, (Human) 57144, (Human) 56924
mitotic cell cycle
regulation of transcription, DNA-templated
apoptotic process
cytoskeleton organization
signal transduction by protein phosphorylation
cell-cell adhesion
movement of cell or subcellular component
signal transduction
cell proliferation
cell growth
cell migration
dendritic spine development
cellular response to organic cyclic compound
learning
memory
locomotory behavior
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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