Invitrogen

Phospho-PDGFRA (Tyr754) Polyclonal Antibody

2 References
View all (78) PDGFRA (CD140a) antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

441008G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PDGFRa that contains tyrosine 754.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533552

Product Specific Information

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFRA. The final product is generated by affinity chromatography using a PDGFRA-derived peptide phosphorylated at tyrosine 754.

Human, rat (100% homologous), and frog (92%) PDGFRA have not been tested, but are expected to react. PDGFRB (50%) has not been tested, but is not expected to react. Positive controls used with this antibody in western blotting were NIH3T3 cells +/- PDGF.

Target Information

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; PDGFR-2; pdgfr-a; PDGFR-alpha; PDGFRA/BCR fusion; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein

View more View less

Gene Aliases: AI115593; APDGFR; CD140A; GAS9; PDGFACE; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA

View more View less

UniProt ID: (Human) P16234, (Rat) P20786, (Mouse) P26618

View more View less

Entrez Gene ID: (Human) 5156, (Rat) 25267, (Mouse) 18595

View more View less

Function(s)
protein kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding ATP binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity platelet-derived growth factor binding protein complex binding phosphatidylinositol 3-kinase binding protein dimerization activity nucleotide binding protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
luteinization in utero embryonic development hematopoietic progenitor cell differentiation positive regulation of cytosolic calcium ion concentration estrogen metabolic process positive regulation of cell proliferation female gonad development anatomical structure morphogenesis response to hormone organ morphogenesis response to organic substance response to inorganic substance negative regulation of platelet activation positive regulation of phospholipase C activity cell migration peptidyl-tyrosine phosphorylation signal transduction involved in regulation of gene expression extracellular matrix organization lung development adrenal gland development positive regulation of cell migration male genitalia development response to estradiol Leydig cell differentiation response to cytokine platelet-derived growth factor receptor-alpha signaling pathway cellular response to platelet-derived growth factor stimulus vascular endothelial growth factor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway wound healing gliogenesis odontogenesis of dentin-containing tooth positive regulation of phosphatidylinositol 3-kinase activity response to estrogen positive regulation of DNA replication protein autophosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis skeletal system morphogenesis inner ear development regulation of chemotaxis cardiac myofibril assembly response to hyperoxia palate development face morphogenesis cell chemotaxis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to amino acid stimulus metanephric glomerular capillary formation regulation of mesenchymal stem cell differentiation protein phosphorylation chemotaxis transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development phosphorylation cellular response to reactive oxygen species MAPK cascade cell activation regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling viral process positive regulation of GTPase activity phosphatidylinositol phosphorylation regulation of actin cytoskeleton reorganization
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support