Invitrogen

Phospho-PDX1 (Ser66) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-118709

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PDX1(Accession P52945), corresponding to amino acid residues around phosphorylated Ser66.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2903209

Product Specific Information

Antibody detects endogenous levels of PDX1 only when phosphorylated at Ser66.

Target Information

The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DLDBP; E3BP; Glucose-sensitive factor; GSF; IDX-1; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; insulin promotor factor 1; Insulin upstream factor 1; IPF-1; Islet/duodenum homeobox 1; Islet/duodenum homeobox-1; IUF-1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox gene 1; pancreatic-duodenal homeobox factor 1; PDHX; PDX-1; somatostatin transactivating factor 1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1

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Gene Aliases: GSF; IDX-1; Idx1; IPF-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; PDX1; STF-1; STF1

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UniProt ID: (Human) P52945, (Rat) P52947, (Mouse) P52946

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Entrez Gene ID: (Human) 3651, (Rat) 29535, (Mouse) 18609

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor binding protein complex binding protein heterodimerization activity DNA binding protein binding sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter liver development type B pancreatic cell differentiation glucose metabolic process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter smoothened signaling pathway nitric oxide mediated signal transduction central nervous system development positive regulation of cell proliferation negative regulation of cell proliferation response to wounding response to glucose response to iron(II) ion response to chlorate organ senescence positive regulation of cell death response to organic cyclic compound morphogenesis of embryonic epithelium insulin secretion cell differentiation pancreas development exocrine pancreas development endocrine pancreas development organ regeneration response to nutrient levels positive regulation of insulin secretion response to vitamin response to lipid response to cytokine response to nicotine positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation response to drug glucose homeostasis response to leucine response to alkaloid positive regulation of DNA binding negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development stem cell differentiation response to glucocorticoid detection of glucose transdifferentiation response to fatty acid negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of type B pancreatic cell apoptotic process multicellular organism development regulation of gene expression generation of precursor metabolites and energy organ morphogenesis
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