Invitrogen

Phospho-PEA15 (Ser116) Polyclonal Antibody

Phospho-PEA15 (Ser116) Antibody in Western Blot (WB)

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Phospho-PEA15 (Ser116) Antibody (44-836G) in WB

Western blot analysis was performed on whole cell extracts (20 µg lysate) of L6 (lane 1) and L6 treated for 10 minutes with 200 ng/mL of Insulin (lane 2). The blots were probed with Anti-PEA-15 (pS116) Rabbit Polyclonal Antibody (Product # 44-836G, 1:500-1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Recombinant Superclonal™ Secondary Antibody, HRP conjugate (Pro... View More

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Product Details

44-836G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PED/PEA-15 that contains serine 116. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533775

Target Information

PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrated Molecular Characterization of Uterine Carcinosarcoma.

Cancer cell

Cherniack AD,Shen H,Walter V,Stewart C,Murray BA,Bowlby R,Hu X,Ling S,Soslow RA,Broaddus RR,Zuna RE,Robertson G,Laird PW,Kucherlapati R,Mills GB,Weinstein JN,Zhang J,Akbani R,Levine DA

44836G was used in reverse phase protein array to characterize the molecular identity of uterine carcinosarcomas.

Mon Mar 13 00:00:00 UTC 2017

Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling.

Cell systems

Hill SM,Nesser NK,Johnson-Camacho K,Jeffress M,Johnson A,Boniface C,Spencer SE,Lu Y,Heiser LM,Lawrence Y,Pande NT,Korkola JE,Gray JW,Mills GB,Mukherjee S,Spellman PT

44836G was used in western blot to analyze the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches

Wed Jan 25 00:00:00 UTC 2017

The protein phosphatase 4 - PEA15 axis regulates the survival of breast cancer cells.

Cellular signalling

Mohammed HN,Pickard MR,Mourtada-Maarabouni M

44-836G was used in western blot to elucidate regulation of the survival of breast cancer cells by the protein phosphatase 4-PEA15 axis

Thu Sep 01 00:00:00 UTC 2016

Effects of anti-depressant treatments on FADD and p-FADD protein in rat brain cortex: enhanced anti-apoptotic p-FADD/FADD ratio after chronic desipramine and fluoxetine administration.

Psychopharmacology

García-Fuster MJ,García-Sevilla JA

44-836G was used in western blot to study enhanced anti-apoptotic p-FADD/FADD ratio after chronic desipramine and fluoxetine administration as anti-depressant treatments in rat brain cortex

Mon Aug 01 00:00:00 UTC 2016

The neuroplastic index p-FADD/FADD and phosphoprotein PEA-15, interacting at GABAA receptor, are upregulated in brain cortex during midazolam-induced hypnosis in mice.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology

Álvaro-Bartolomé M,García-Sevilla JA

44-836G was used in western blot to test if FADD participates in the activation of ligand-gated ion channels.

Sun Nov 01 00:00:00 UTC 2015

Regulation of hippocampal Fas receptor and death-inducing signaling complex after kainic acid treatment in mice.

Progress in neuro-psychopharmacology & biological psychiatry

Keller B,García-Sevilla JA

44-836G was used in western blot to investigate neuronal death in the hippocampus.

Thu Dec 03 00:00:00 UTC 2015

FADD adaptor and PEA-15/ERK1/2 partners in major depression and schizophrenia postmortem brains: basal contents and effects of psychotropic treatments.

Neuroscience

García-Fuster MJ,Díez-Alarcia R,Ferrer-Alcón M,La Harpe R,Meana JJ,García-Sevilla JA

44-836G was used in western blot to investigate Fas-associated death domain in patients with major depression and schizophrenia

Fri Sep 26 00:00:00 UTC 2014

CDKN2D-WDFY2 is a cancer-specific fusion gene recurrent in high-grade serous ovarian carcinoma.

PLoS genetics

Kannan K,Coarfa C,Rajapakshe K,Hawkins SM,Matzuk MM,Milosavljevic A,Yen L

44-836G was used in Western Blotting to identify a cancer-specific and inter-chromosomal fusion gene that's recurrent in high-grade serous ovarian carcinoma.

Sat Mar 01 00:00:00 UTC 2014

Molecular adaptations of apoptotic pathways and signaling partners in the cerebral cortex of human cocaine addicts and cocaine-treated rats.

Neuroscience

Alvaro-Bartolomé M,La Harpe R,Callado LF,Meana JJ,García-Sevilla JA

Thu Nov 24 00:00:00 UTC 2011

Molecular adaptations of apoptotic pathways and signaling partners in the cerebral cortex of human cocaine addicts and cocaine-treated rats.

Neuroscience

Alvaro-Bartolomé M,La Harpe R,Callado LF,Meana JJ,García-Sevilla JA

Thu Nov 24 00:00:00 UTC 2011

The time course of unconditioned morphine-induced psychomotor sensitization mirrors the phosphorylation of FADD and MEK/ERK in rat striatum: role of PEA-15 as a FADD-ERK binding partner in striatal plasticity.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology

Ramos-Miguel A,Esteban S,García-Sevilla JA

Fri Jan 01 00:00:00 UTC 2010

Protein phosphatase 4 regulates apoptosis, proliferation and mutation rate of human cells.

Biochimica et biophysica acta

Mourtada-Maarabouni M,Williams GT

Fri Aug 01 00:00:00 UTC 2008

Phosphorylation of PEA-15 switches its binding specificity from ERK/MAPK to FADD.

The Biochemical journal

Renganathan H,Vaidyanathan H,Knapinska A,Ramos JW

Thu Sep 15 00:00:00 UTC 2005

Phosphorylation of PEA-15 switches its binding specificity from ERK/MAPK to FADD.

The Biochemical journal

Renganathan H,Vaidyanathan H,Knapinska A,Ramos JW

Thu Sep 15 00:00:00 UTC 2005

Phosphorylation of PEA-15 switches its binding specificity from ERK/MAPK to FADD.

The Biochemical journal

Renganathan H,Vaidyanathan H,Knapinska A,Ramos JW

Thu Sep 15 00:00:00 UTC 2005

Phosphorylation of PEA-15 switches its binding specificity from ERK/MAPK to FADD.

The Biochemical journal

Renganathan H,Vaidyanathan H,Knapinska A,Ramos JW

Thu Sep 15 00:00:00 UTC 2005

Phosphorylation of phosphoprotein enriched in astrocytes (PEA-15) regulates extracellular signal-regulated kinase-dependent transcription and cell proliferation.

Molecular biology of the cell

Krueger J,Chou FL,Glading A,Schaefer E,Ginsberg MH

Mon Aug 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: 15 kDa phosphoprotein enriched in astrocytes; Astrocytic phosphoprotein PEA-15; homolog of mouse MAT-1 oncogene; mammary transforming gene 1; mammary transforming gene 1, mouse, homolog of; OTTHUMP00000029887; PED; phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in diabetes

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Gene Aliases: HMAT1; HUMMAT1H; MAT1; MAT1H; PEA-15; PEA15; Pea15a; PED; Pkcs15

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UniProt ID: (Human) Q15121, (Mouse) Q62048

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Entrez Gene ID: (Human) 8682, (Mouse) 18611

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Function(s)

protein binding protein kinase C binding

Process(es)

DNA damage checkpoint MAPK cascade activation of MAPK activity transport apoptotic process carbohydrate transport response to morphine negative regulation of glucose import negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of extrinsic apoptotic signaling pathway via death domain receptors intracellular signal transduction regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-PEA15 (Ser116) Polyclonal Antibody

Phospho-PEA15 (Ser116) Antibody (44-836G) in WB

Product Details

44-836G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Dot blot (DB)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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