Invitrogen

Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4)

Phospho-PERK (Thr980) Antibody in Western Blot (WB)

FIGURE: 1 / 20

Phospho-PERK (Thr980) Antibody (MA5-15033) in WB

Western blot was performed using anti-Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4) (Product # MA5-15033) and a 125 kDa band corresponding to Phospho-PERK was induced in RSC96 cells upon thapsigargin and tunicamycin treatments which are known to induce ER stress. Whole cell extracts (30 µg lysate) of RSC96 (Lane1), RSC96 treated with Thapsigargin (300nM for 2hrs) (Lane 2) and RSC96 with Tunicamycin (4... View More

Phospho-PERK (Thr980) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA5-15033

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.305.4

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr980 of mouse PERK

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

441 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10980432

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Beclin-1 dependent autophagy improves renal outcomes following Unilateral Ureteral Obstruction (UUO) injury.

Frontiers in immunology

Lopez-Soler RI,Nikouee A,Kim M,Khan S,Sivaraman L,Ding X,Zang QS

MA5-15033 was used in Western Blot, Immunohistochemistry to suggest that Unilateral Ureteral Obstruction (UUO) causes an insufficient, maladaptive renal autophagy, which triggered downstream activation of inflammatory STING pathway, production of cytokines, and pathological activation of ISR, eventually leading to the development of fibrosis.

Tue Mar 07 00:00:00 UTC 2023

Beclin-1 dependent autophagy improves renal outcomes following Unilateral Ureteral Obstruction (UUO) injury.

Frontiers in immunology

Lopez-Soler RI,Nikouee A,Kim M,Khan S,Sivaraman L,Ding X,Zang QS

Tue Mar 07 00:00:00 UTC 2023

Intracellular alpha-fetoprotein mitigates hepatocyte apoptosis and necroptosis by inhibiting endoplasmic reticulum stress.

World journal of gastroenterology

Chen YF,Liu SY,Cheng QJ,Wang YJ,Chen S,Zhou YY,Liu X,Jiang ZG,Zhong WW,He YH

Published figure using Phospho-PERK (Thr980) monoclonal antibody (Product # MA5-15033) in Western Blot

Thu Jul 14 00:00:00 UTC 2022

Four-dimensional nuclear speckle phase separation dynamics regulate proteostasis.

Science advances

Dion W,Ballance H,Lee J,Pan Y,Irfan S,Edwards C,Sun M,Zhang J,Zhang X,Liu S,Zhu B

MA5-15033 was used in Western Blot to show an evolutionarily conserved XBP1s-SON axis that establishes a cell-autonomous mammalian 12-hour ultradian rhythm of nuclear speckle liquid-liquid phase separation dynamics, separate from both the 24-hour circadian clock and the cell cycle.

Fri Jan 07 00:00:00 UTC 2022

Four-dimensional nuclear speckle phase separation dynamics regulate proteostasis.

Science advances

Dion W,Ballance H,Lee J,Pan Y,Irfan S,Edwards C,Sun M,Zhang J,Zhang X,Liu S,Zhu B

Fri Jan 07 00:00:00 UTC 2022

Schweinfurthin induces ICD without ER stress and caspase activation.

Oncoimmunology

Zhang R,Neighbors JD,Schell TD,Hohl RJ

MA5-15033 was used in Western Blotting to investigate the mechanisms by which MeSG treatment induces immunogenic cell death (ICD).

Tue Aug 09 00:00:00 UTC 2022

Schweinfurthin induces ICD without ER stress and caspase activation.

Oncoimmunology

Zhang R,Neighbors JD,Schell TD,Hohl RJ

MA5-15033 was used in Western Blotting to investigate the mechanisms by which MeSG treatment induces immunogenic cell death (ICD).

Tue Aug 09 00:00:00 UTC 2022

Dapagliflozin attenuates pressure overload-induced myocardial remodeling in mice via activating SIRT1 and inhibiting endoplasmic reticulum stress.

Acta pharmacologica Sinica

Ren FF,Xie ZY,Jiang YN,Guan X,Chen QY,Lai TF,Li L

MA5-15033 was used in Western Blotting to indicate that DAPA could inhibit the PERK-eIF2α-CHOP axis of the ER stress response through the activation of SIRT1 in Ang II-treated cardiomyocytes and ameliorate heart failure development in vivo.

Fri Jul 01 00:00:00 UTC 2022

High-fat diet aggravates colitis via mesenteric adipose tissue derived exosome metastasis-associated lung adenocarcinoma transcript 1.

World journal of gastroenterology

Chen D,Lu MM,Wang JH,Ren Y,Xu LL,Cheng WX,Wang SS,Li XL,Cheng XF,Gao JG,Kalyani FS,Jin X

MA5-15033 was used in Inhibition Assays to study the role of mesenteric adipose tissue-derived exosomes on developing Crohn's disease.

Sun Aug 07 00:00:00 UTC 2022

Spata7 is required for maintenance of the retinal connecting cilium.

Scientific reports

Lu J,Xiong K,Qian X,Choi J,Shim YK,Burnett J,Mardon G,Chen R

MA5-15033 was used in Immunohistochemistry to indicate that Spata7 is required not only for the establishment but also for the maintenance of the CC of photoreceptors.

Sat Apr 02 00:00:00 UTC 2022

Albendazole reduces hepatic inflammation and endoplasmic reticulum-stress in a mouse model of chronic Echinococcus multilocularis infection.

PLoS neglected tropical diseases

Weingartner M,Stücheli S,Jebbawi F,Gottstein B,Beldi G,Lundström-Stadelmann B,Wang J,Odermatt A

MA5-15033 was used in Western Blot to study the involvement of unfolded protein response and endoplasmic reticulum-stress during E. multilocularis infection in mice.

Sat Jan 01 00:00:00 UTC 2022

Schizandrin A induces the apoptosis and suppresses the proliferation, invasion and migration of gastric cancer cells by activating endoplasmic reticulum stress.

Molecular medicine reports

Pu H,Qian Q,Wang F,Gong M,Ge X

MA5-15033 was used in Western Blotting to identify how schizandrin A functioned to affect the progression of gastric cancer.

Mon Nov 01 00:00:00 UTC 2021

Schizandrin A induces the apoptosis and suppresses the proliferation, invasion and migration of gastric cancer cells by activating endoplasmic reticulum stress.

Molecular medicine reports

Pu H,Qian Q,Wang F,Gong M,Ge X

MA5-15033 was used in Western Blotting to identify how schizandrin A functioned to affect the progression of gastric cancer.

Mon Nov 01 00:00:00 UTC 2021

Inhibition of ERAD synergizes with FTS to eradicate pancreatic cancer cells.

BMC cancer

Du R,Sullivan DK,Azizian NG,Liu Y,Li Y

MA5-15033 was used in Western Blotting to reveal a critical role for ERAD in therapeutic response of FTS and points to the modulation of UPR as a novel approach to improve the efficacy of FTS in PDAC treatment.

Sat Mar 06 00:00:00 UTC 2021

Inhibition of ERAD synergizes with FTS to eradicate pancreatic cancer cells.

BMC cancer

Du R,Sullivan DK,Azizian NG,Liu Y,Li Y

Sat Mar 06 00:00:00 UTC 2021

Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons.

EBioMedicine

Arioka Y,Shishido E,Kushima I,Suzuki T,Saito R,Aiba A,Mori D,Ozaki N

MA5-15033 was used in Western Blotting to conclude that the 22q11.2 deletion causes various vulnerabilities in dopaminergic neurons, dependent on PERK dysfunction.

Fri Jan 01 00:00:00 UTC 2021

Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons.

EBioMedicine

Arioka Y,Shishido E,Kushima I,Suzuki T,Saito R,Aiba A,Mori D,Ozaki N

MA5-15033 was used in Western Blotting to conclude that the 22q11.2 deletion causes various vulnerabilities in dopaminergic neurons, dependent on PERK dysfunction.

Fri Jan 01 00:00:00 UTC 2021

Leptin Induces Apoptotic and Pyroptotic Cell Death via NLRP3 Inflammasome Activation in Rat Hepatocytes.

International journal of molecular sciences

Baral A,Park PH

MA5-15033 was used in Western Blot to explore the mechanism of leptin in the pathophysiology of liver diseases, including inflammasomes activation, using a rat model.

Mon Nov 22 00:00:00 UTC 2021

Leptin Induces Apoptotic and Pyroptotic Cell Death via NLRP3 Inflammasome Activation in Rat Hepatocytes.

International journal of molecular sciences

Baral A,Park PH

MA5-15033 was used in Western Blot to explore the mechanism of leptin in the pathophysiology of liver diseases, including inflammasomes activation, using a rat model.

Mon Nov 22 00:00:00 UTC 2021

Inhibition of TGFβ-activated protein kinase 1 ameliorates myocardial ischaemia/reperfusion injury via endoplasmic reticulum stress suppression.

Journal of cellular and molecular medicine

Zeng J,Jin Q,Ruan Y,Sun C,Xu G,Chu M,Ji K,Wu L,Li L

MA5-15033 was used in Western Blotting to present the first direct evidence that inhibition of TAK1 mitigated MI/R injury, and TAK1 mediated ROS/ER stress/apoptosis signal pathway is important for the pathogenesis of MI/R injury.

Mon Jun 01 00:00:00 UTC 2020

Inhibition of TGFβ-activated protein kinase 1 ameliorates myocardial ischaemia/reperfusion injury via endoplasmic reticulum stress suppression.

Journal of cellular and molecular medicine

Zeng J,Jin Q,Ruan Y,Sun C,Xu G,Chu M,Ji K,Wu L,Li L

Mon Jun 01 00:00:00 UTC 2020

Inhibition of USP14 induces ER stress-mediated autophagy without apoptosis in lung cancer cell line A549.

Cell stress & chaperones

Moghadami AA,Aboutalebi Vand Beilankouhi E,Kalantary-Charvadeh A,Hamzavi M,Mosayyebi B,Sedghi H,Ghorbani Haghjo A,Nazari Soltan Ahmad S

MA5-15033 was used in Western Blotting to determine whether the accumulation of ubiquitinated proteins results in endoplasmic reticulum stress-mediated autophagy.

Sun Nov 01 00:00:00 UTC 2020

Swimming Differentially Affects T2DM-Induced Skeletal Muscle ER Stress and Mitochondrial Dysfunction Related to MAM.

Diabetes, metabolic syndrome and obesity : targets and therapy

Zhang Z,Cui D,Zhang T,Sun Y,Ding S

MA5-15033 was used in Western Blotting to show that MAM quality and ATF6 might be novel and important targets for T2DM treatment.

Thu Apr 14 00:00:00 UTC 2022

Swimming Differentially Affects T2DM-Induced Skeletal Muscle ER Stress and Mitochondrial Dysfunction Related to MAM.

Diabetes, metabolic syndrome and obesity : targets and therapy

Zhang Z,Cui D,Zhang T,Sun Y,Ding S

MA5-15033 was used in Western Blotting to show that MAM quality and ATF6 might be novel and important targets for T2DM treatment.

Thu Apr 14 00:00:00 UTC 2022

Inhibition of eIF2α Dephosphorylation Protects Hepatocytes from Apoptosis by Alleviating ER Stress in Acute Liver Injury.

BioMed research international

Tang YJ,Chen H,Yi Y,Chen GM,Yang FW,Li Y,Tian RD,Huang WG,Cheng QJ,He YH

MA5-15033 was used in Western Blotting to investigate the effects of eIF2α phosphorylation on hepatocyte apoptosis and the ER stress mechanisms in acute liver injury.

Mon Mar 22 00:00:00 UTC 2021

Antifungal itraconazole ameliorates experimental autoimmune encephalomyelitis through a novel mechanism of action.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Huang H,Tian X,Peng X,Huang L,Mei L,Zhan Y,Chen S,Wu H,Wei G,Cai X

MA5-15033 was used in Western Blotting to reveal a new method for treating MS using currently approved antifungal drugs.

Fri May 01 00:00:00 UTC 2020

High-fat diet intake modulates maternal intestinal adaptations to pregnancy and results in placental hypoxia, as well as altered fetal gut barrier proteins and immune markers.

The Journal of physiology

Gohir W,Kennedy KM,Wallace JG,Saoi M,Bellissimo CJ,Britz-McKibbin P,Petrik JJ,Surette MG,Sloboda DM

MA5-15033 was used in Western Blotting to suggest that HFD intake prior to and during pregnancy shifts the composition of the maternal gut microbiota and impairs gut barrier integrity, resulting in increased maternal circulating LPS, which may ultimate contribute to changes in placental vascularization and fetal gut development.

Sat Jun 01 00:00:00 UTC 2019

Carbon monoxide-induced TFEB nuclear translocation enhances mitophagy/mitochondrial biogenesis in hepatocytes and ameliorates inflammatory liver injury.

Cell death & disease

Kim HJ,Joe Y,Rah SY,Kim SK,Park SU,Park J,Kim J,Ryu J,Cho GJ,Surh YJ,Ryter SW,Kim UH,Chung HT

MA5-15033 was used in Western Blotting to demonstrate that the activation of protein kinase R-like endoplasmic reticulum kinase with carbon monoxide increases the nuclear translocation of transcription factor EB.

Wed Oct 17 00:00:00 UTC 2018

Mesencephalic astrocyte-derived neurotrophic factor reduces cell apoptosis via upregulating HSP70 in SHSY-5Y cells.

Translational neurodegeneration

Sun H,Jiang M,Fu X,Cai Q,Zhang J,Yin Y,Guo J,Yu L,Jiang Y,Liu Y,Feng L,Nie Z,Fang J,Jin L

MA5-15033 was used in Western Blotting to study the role of HSP70 expression in the mesencephalic astrocyte-derived neurotrophic factor (MANF) - mediated survival pathway.

Wed Nov 20 00:00:00 UTC 2019

HCV induces transforming growth factor β1 through activation of endoplasmic reticulum stress and the unfolded protein response.

Scientific reports

Chusri P,Kumthip K,Hong J,Zhu C,Duan X,Jilg N,Fusco DN,Brisac C,Schaefer EA,Cai D,Peng LF,Maneekarn N,Lin W,Chung RT

MA5-15033 was used in western blot to determine the induction of transforming growth factor beta1 through activation of endoplasmic reticulum stress and the unfolded protein response due to HCV

Tue Mar 01 00:00:00 UTC 2016

Protein tyrosine phosphatase receptor R and Z1 expression as independent prognostic indicators in oral squamous cell carcinoma.

Head & neck

Duś-Szachniewicz K,Woźniak M,Nelke K,Gamian E,Gerber H,Ziółkowski P

MA5-15033 was used in western blot to study the function of PTPRR and PTPRZ1 proteins in oral cavity squamous cell carcinoma

Tue Dec 01 00:00:00 UTC 2015

Legionella suppresses the host unfolded protein response via multiple mechanisms.

Nature communications

Treacy-Abarca S,Mukherjee S

MA5-15033 was used in western blot to examine the role of the host unfolded protein response during L. pneumophila infection

Wed Jul 29 00:00:00 UTC 2015

Legionella suppresses the host unfolded protein response via multiple mechanisms.

Nature communications

Treacy-Abarca S,Mukherjee S

MA5-15033 was used in western blot to examine the role of the host unfolded protein response during L. pneumophila infection

Wed Jul 29 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; Protein tyrosine kinase EIF2AK3

View more View less

Gene Aliases: Eif2ak3; PEK; Perk

View more View less

UniProt ID: (Rat) Q9Z1Z1

View more View less

Entrez Gene ID: (Rat) 29702

View more View less

Function(s)

protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity ATP binding enzyme binding protein phosphatase binding identical protein binding

Process(es)

skeletal system development ossification angiogenesis chondrocyte development translation protein phosphorylation activation of cysteine-type endopeptidase activity involved in apoptotic process ER overload response endoplasmic reticulum organization lactation positive regulation of signal transduction positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression regulation of translational initiation by eIF2 alpha phosphorylation negative regulation of translation regulation of fatty acid metabolic process calcium-mediated signaling bone mineralization endoplasmic reticulum unfolded protein response pancreas development endocrine pancreas development negative regulation of myelination negative regulation of translation in response to stress negative regulation of translational initiation in response to stress positive regulation of protein binding SREBP signaling pathway response to endoplasmic reticulum stress cellular response to glucose starvation negative regulation of apoptotic process fat cell differentiation positive regulation of transcription from RNA polymerase I promoter protein autophosphorylation insulin-like growth factor receptor signaling pathway protein homooligomerization intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4)

Phospho-PERK (Thr980) Antibody (MA5-15033) in WB

Product Details

MA5-15033

Western Blot (WB)

Immunohistochemistry (IHC)

Inhibition Assays (IA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!