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          • Primary Antibodies ›
          • PKC Pan Antibodies

          Invitrogen

          Phospho-PKC Pan (Thr497) Polyclonal Antibody

          Advanced Verification
          View all (5) PKC Pan antibodies

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          Cite Phospho-PKC Pan (Thr497) Polyclonal Antibody

          • Antibody Testing Data (4)
          • Advanced Verification (1)
          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Phospho-PKC Pan (Thr497) Antibody (PA5-36757) in IHC (P)

          Immunohistochemistry analysis of PKC Pan in paraffin-embedded human breast carcinoma tissue. Samples were incubated with PKC Pan polyclonal antibody (Product # PA5-36757). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-PKC Pan (Thr497) Antibody in Western Blot (WB)
          Phospho-PKC Pan (Thr497) Antibody in Western Blot (WB)
          Phospho-PKC Pan (Thr497) Antibody

          Product Details

          PA5-36757

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2553707

          Product Specific Information

          This antibody detects endogenous levels of PKC alpha, beta-I, beta-II, gamma, delta, epsilon, eta, zeta and theta isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKC alpha.

          Purity is >95% by SDS-PAGE.

          Target Information

          Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ( alpha, betaI, betaII and gamma) and novel (n) PKC isoforms ( delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 14 - 3 - 3 - zeta isoform; aging-associated gene 6; aPKC-lambda/iota; atypical protein kinase C; Atypical protein kinase C-lambda/iota; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PKC theta; PKC zeta; PKC-A; PKC-alpha; PKC-B; PKC-beta; PKC-gamma; PKC-L; PRKC-lambda/iota; Protein kinase C alpha type; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; Protein kinase C delta type; protein kinase C delta variant IX; protein kinase C delta VIII; Protein kinase C epsilon type; Protein kinase C eta type; Protein kinase C gamma type; Protein kinase C iota type; Protein kinase C theta type; protein kinase C type I (gamma type); protein kinase C zeta subspecies; Protein kinase C zeta type; protein kinase C, alpha; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C, epsilon; protein kinase C, eta; protein kinase C, gamma; protein kinase C, iota; protein kinase C, lambda; protein kinase C, theta; protein kinase C, zeta; protein kinase c-alpha; protein kinase C-eta; protein kinase C[d]; Tyrosine-protein kinase PRKCD

          View more View less

          Gene Aliases: 14-3-3-zetaisoform; 2310021H13Rik; 5830406C15Rik; A130035A12Rik; A130082F03Rik; AAG6; AI098070; AI385711; AI427505; AI875142; ALPS3; aPKClambda; aPKCzeta; AW494342; C80388; CVID9; D14Ertd420e; DXS1179E; MAY1; mKIAA4165; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PKC; PKC-0; PKC-alpha; PKC-Beta; PKC-gamma; PKC-L; PKC-theta; PKC-ZETA; PKC2; PKC[d]; PKC[e]; PKCA; PKCB; PKCC; PKCD; PKCdelta; PKCE; Pkcea; PKCepsilon; PKCG; PKCgamma; Pkch; PKCI; PKCL; PKClambda; Pkcq; PKCtheta; Pkcz; PRKACA; Prkc; PRKCA; PRKCB; PRKCB1; PRKCB2; Prkcc; PRKCD; PRKCE; PRKCG; PRKCH; PRKCI; PRKCL; PRKCQ; PRKCT; PRKCZ; r14-3-3; R74924; R75156; RATPKCI; SCA14; zetaPKC

          View more View less

          UniProt ID: (Human) P17252, (Human) P05771, (Human) Q05655, (Human) Q02156, (Human) P05129, (Human) P24723, (Human) P41743, (Human) Q04759, (Human) Q05513, (Mouse) P20444, (Mouse) P68404, (Rat) P68403, (Mouse) P28867, (Rat) P09215, (Mouse) P16054, (Rat) P09216, (Mouse) P63318, (Rat) P63319, (Mouse) P23298, (Rat) Q64617, (Mouse) Q62074, (Rat) F1M7Y5, (Mouse) Q02111, (Rat) P09217, (Mouse) Q02956

          View more View less

          Entrez Gene ID: (Human) 5578, (Human) 5579, (Human) 5580, (Human) 5581, (Human) 5582, (Human) 5583, (Human) 5584, (Human) 5588, (Human) 5590, (Rat) 24680, (Mouse) 18750, (Mouse) 18751, (Rat) 25023, (Mouse) 18753, (Rat) 170538, (Mouse) 18754, (Rat) 29340, (Mouse) 18752, (Rat) 24681, (Mouse) 18755, (Rat) 81749, (Mouse) 18759, (Rat) 84006, (Rat) 85420, (Mouse) 18761, (Rat) 25522, (Mouse) 18762

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein binding ATP binding zinc ion binding enzyme binding histone kinase activity (H3-T6 specific) chromatin binding protein kinase C binding calcium channel regulator activity ligand-dependent nuclear receptor transcription coactivator activity histone binding androgen receptor binding calcium-independent protein kinase C activity non-membrane spanning protein tyrosine kinase activity enzyme activator activity kinase binding protein kinase binding insulin receptor substrate binding metal ion binding actin monomer binding signal transducer activity receptor activator activity ethanol binding 14-3-3 protein binding protein serine/threonine/tyrosine kinase activity Ral GTPase binding phospholipid binding protein domain specific binding potassium channel regulator activity phospholipase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups TIR domain binding receptor binding SH3 domain binding molecular_function
          Process(es)
          response to hypoxia immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport cellular calcium ion homeostasis apoptotic process negative regulation of glucose transport regulation of dopamine secretion phosphorylation chromatin modification positive regulation of vascular endothelial growth factor receptor signaling pathway histone H3-T6 phosphorylation intracellular signal transduction regulation of growth B cell activation response to drug positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to carbohydrate stimulus response to oxidative stress cell cycle aspartate transport immunoglobulin mediated immune response peptidyl-threonine phosphorylation termination of signal transduction negative regulation of actin filament polymerization interleukin-10 production interleukin-12 production positive regulation of superoxide anion generation regulation of actin cytoskeleton organization collagen metabolic process negative regulation of glial cell apoptotic process cellular response to oxidative stress positive regulation of protein dephosphorylation B cell proliferation neutrophil activation regulation of phosphorylation defense response to bacterium positive regulation of apoptotic process positive regulation of MAP kinase activity negative regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of glucose import protein autophosphorylation negative regulation of peptidyl-tyrosine phosphorylation negative regulation of filopodium assembly negative regulation of platelet aggregation cellular senescence positive regulation of phospholipid scramblase activity positive regulation of ceramide biosynthetic process positive regulation of glucosylceramide catabolic process positive regulation of sphingomyelin catabolic process positive regulation of response to DNA damage stimulus positive regulation of apoptotic signaling pathway positive regulation of macrophage derived foam cell differentiation cell differentiation positive regulation of keratinocyte differentiation positive regulation of glial cell proliferation protein kinase C signaling regulation of bicellular tight junction assembly inactivation of MAPK activity response to reactive oxygen species angiogenesis negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of the force of heart contraction chondrocyte differentiation desmosome assembly negative regulation of protein kinase activity regulation of muscle contraction cell adhesion aging learning or memory negative regulation of cell proliferation response to mechanical stimulus response to toxic substance positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy response to organic cyclic compound negative regulation of translation peptidyl-serine phosphorylation central nervous system neuron axonogenesis positive regulation of cell migration neutrophil chemotaxis positive regulation of lipopolysaccharide-mediated signaling pathway response to estradiol peptidyl-serine autophosphorylation response to peptide hormone positive regulation of blood vessel endothelial cell migration establishment of protein localization response to ethanol positive regulation of macrophage differentiation positive regulation of cell adhesion negative regulation of heart contraction positive regulation of exocytosis positive regulation of mitotic cell cycle negative regulation of glucose import response to antibiotic regulation of receptor-mediated endocytosis positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis response to corticosterone positive regulation of synapse assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 regulation of platelet aggregation intrinsic apoptotic signaling pathway positive regulation of dense core granule biogenesis membrane protein ectodomain proteolysis inflammatory response response to heat response to glucose regulation of G2/M transition of mitotic cell cycle regulation of vasoconstriction neuron differentiation positive regulation of interleukin-17 production positive regulation of interleukin-4 production response to insulin positive regulation of T cell proliferation tissue regeneration positive regulation of NF-kappaB import into nucleus positive regulation of interleukin-2 biosynthetic process respiratory burst positive regulation of protein secretion positive regulation of T cell activation positive regulation of filopodium assembly positive regulation of stress fiber assembly negative regulation of T cell apoptotic process positive regulation of T-helper 17 type immune response positive regulation of T-helper 2 cell activation macrophage activation involved in immune response signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to morphine positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation adaptive immune response mitotic nuclear envelope disassembly lipoprotein transport actin filament organization multicellular organism development positive regulation of neuron projection development cell migration cellular response to insulin stimulus cellular protein localization establishment of apical/basal cell polarity eye photoreceptor cell development negative regulation of neuron apoptotic process cell-cell junction organization Golgi vesicle budding positive regulation of establishment of protein localization to plasma membrane positive regulation of endothelial cell apoptotic process response to organonitrogen compound cellular response to glucose starvation response to hydrogen peroxide positive regulation of catalytic activity response to amino acid dibenzo-p-dioxin metabolic process response to vitamin D positive regulation of odontogenesis of dentin-containing tooth chemical synaptic transmission chemosensory behavior regulation of response to food positive regulation of mismatch repair negative regulation of protein catabolic process regulation of circadian rhythm response to pain rhythmic process regulation of phagocytosis innervation negative regulation of proteasomal protein catabolic process negative regulation of mitochondrial membrane potential regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration microtubule cytoskeleton organization positive regulation of cell-matrix adhesion transforming growth factor beta receptor signaling pathway long-term memory positive regulation of cell proliferation insulin receptor signaling pathway establishment of cell polarity negative regulation of protein complex assembly actin cytoskeleton reorganization activation of phospholipase D activity activation of protein kinase B activity positive regulation of T-helper 2 cell differentiation positive regulation of insulin receptor signaling pathway vesicle transport along microtubule protein heterooligomerization negative regulation of hydrolase activity membrane depolarization membrane hyperpolarization long-term synaptic potentiation protein localization to plasma membrane neuron projection extension positive regulation of excitatory postsynaptic potential positive regulation of T-helper 2 cell cytokine production positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of interleukin-10 secretion regulation of cell growth cellular component disassembly involved in execution phase of apoptosis axon guidance positive regulation of telomere maintenance via telomerase Fc-epsilon receptor signaling pathway T cell receptor signaling pathway positive regulation of telomerase activity cell chemotaxis positive regulation of telomere capping cell surface receptor signaling pathway positive regulation of synaptic transmission positive regulation of protein transport stimulatory C-type lectin receptor signaling pathway intrinsic apoptotic signaling pathway in response to oxidative stress regulation of receptor activity histone phosphorylation peptidyl-tyrosine phosphorylation positive regulation of endodeoxyribonuclease activity negative regulation of protein binding activation of protein kinase activity positive regulation of protein import into nucleus regulation of mRNA stability negative regulation of inflammatory response protein stabilization interferon-gamma-mediated signaling pathway cellular response to hydrogen peroxide cellular response to hydroperoxide cellular response to angiotensin protein targeting to membrane cytoskeleton organization vesicle-mediated transport establishment or maintenance of epithelial cell apical/basal polarity secretion membrane organization bicellular tight junction assembly activation of adenylate cyclase activity negative regulation of adenylate cyclase activity regulation of insulin secretion apoptotic signaling pathway
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