Invitrogen

Phospho-PKC alpha (Thr638) Recombinant Rabbit Monoclonal Antibody (JF0964)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32506

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF0964

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr638 of Human PKC alpha aa 622-667
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809783

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; Protein kinase C alpha type; protein kinase C, alpha; protein kinase c-alpha

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Gene Aliases: AAG6; AI875142; PKC-alpha; PKCA; PRKACA; PRKCA

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UniProt ID: (Human) P17252, (Mouse) P20444

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Entrez Gene ID: (Human) 5578, (Mouse) 18750, (Rat) 24680

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein binding ATP binding zinc ion binding enzyme binding histone kinase activity (H3-T6 specific) nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding
Process(es)
inactivation of MAPK activity response to reactive oxygen species angiogenesis negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of the force of heart contraction chondrocyte differentiation desmosome assembly protein phosphorylation negative regulation of protein kinase activity cellular calcium ion homeostasis apoptotic process regulation of muscle contraction cell adhesion learning or memory negative regulation of cell proliferation positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy phosphorylation negative regulation of translation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation central nervous system neuron axonogenesis positive regulation of cell migration neutrophil chemotaxis positive regulation of lipopolysaccharide-mediated signaling pathway response to estradiol negative regulation of glial cell apoptotic process histone H3-T6 phosphorylation intracellular signal transduction peptidyl-serine autophosphorylation positive regulation of blood vessel endothelial cell migration response to ethanol positive regulation of macrophage differentiation positive regulation of angiogenesis positive regulation of cell adhesion negative regulation of heart contraction positive regulation of exocytosis positive regulation of mitotic cell cycle negative regulation of glucose import negative regulation of insulin receptor signaling pathway protein autophosphorylation regulation of receptor-mediated endocytosis positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis positive regulation of synapse assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to carbohydrate stimulus regulation of platelet aggregation intrinsic apoptotic signaling pathway positive regulation of dense core granule biogenesis aging response to mechanical stimulus response to toxic substance response to organic cyclic compound response to peptide hormone establishment of protein localization response to antibiotic response to corticosterone mitotic nuclear envelope disassembly activation of adenylate cyclase activity negative regulation of adenylate cyclase activity platelet activation regulation of mRNA stability regulation of insulin secretion apoptotic signaling pathway
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