Invitrogen

Phospho-PKC alpha/beta-2 (Thr638, Thr641) Recombinant Rabbit Monoclonal Antibody (8D8L5)

View all (3) PKC alpha/beta-2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-55521

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:7,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8D8L5

Immunogen

A synthetic phosphorylated peptide around T638 of human PRKCA.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.69 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

RRID

AB_3667760

Target Information

Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aging-associated gene 6; PKC-A; PKC-B; PRKC Pan; Protein kinase C alpha type; Protein kinase C beta type; protein kinase C, alpha; protein kinase C, beta 1 polypeptide

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Gene Aliases: AAG6; PKC-alpha; PKC-beta; PKCA; PKCB; PRKACA; PRKCA; PRKCB; PRKCB1; PRKCB2

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UniProt ID: (Human) P17252, (Human) P05771

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Entrez Gene ID: (Human) 5578, (Human) 5579

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein binding ATP binding zinc ion binding enzyme binding histone kinase activity (H3-T6 specific) chromatin binding protein kinase C binding calcium channel regulator activity ligand-dependent nuclear receptor transcription coactivator activity histone binding androgen receptor binding
Process(es)
response to hypoxia adaptive immune response transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport cellular calcium ion homeostasis apoptotic process mitotic nuclear envelope disassembly signal transduction negative regulation of glucose transport peptidyl-serine phosphorylation platelet activation positive regulation of vascular endothelial growth factor receptor signaling pathway histone H3-T6 phosphorylation intracellular signal transduction B cell activation lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to carbohydrate stimulus angiogenesis positive regulation of endothelial cell proliferation desmosome assembly cell adhesion activation of adenylate cyclase activity negative regulation of adenylate cyclase activity positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy positive regulation of cell migration positive regulation of lipopolysaccharide-mediated signaling pathway negative regulation of glial cell apoptotic process regulation of mRNA stability positive regulation of blood vessel endothelial cell migration positive regulation of macrophage differentiation positive regulation of cell adhesion positive regulation of mitotic cell cycle regulation of insulin secretion positive regulation of ERK1 and ERK2 cascade response to interleukin-1 regulation of platelet aggregation apoptotic signaling pathway positive regulation of dense core granule biogenesis
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