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          • Primary Antibodies ›
          • PKC delta/theta Antibodies

          Invitrogen

          Phospho-PKC delta/theta (Ser643, Ser676) Polyclonal Antibody

          View all (3) PKC delta/theta antibodies

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          Cite Phospho-PKC delta/theta (Ser643, Ser676) Polyclonal Antibody

          • Antibody Testing Data (1)
          Phospho-PKC delta/theta (Ser643, Ser676) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-PKC delta/theta (Ser643, Ser676) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-PKC delta/theta (Ser643, Ser676) Antibody (PA5-110147) in WB

          Western blot analysis of Phospho-PKC delta/theta (Ser643, Ser676) in extracts of RAW264.7 cells. Samples were incubated with Phospho-PKC delta/theta (Ser643, Ser676) Polyclonal antibody (Product # PA5-110147) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes. Lysates/proteins: 25 µ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-PKC delta/theta (Ser643, Ser676) Antibody in Western Blot (WB)

          Product Details

          PA5-110147

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic phosphorylated peptide around S643 of human PKCδ/θPRKCD (NP_0013416051)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.24 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          RRID

          AB_2855558

          Product Specific Information

          Immunogen sequence: RLSYS

          Target Information

          PRKCD (Protein Kinase C Delta) is a Protein Coding gene. Diseases associated with PRKCD includeAutoimmune Lymphoproliferative Syndrome, Type IiiandSystemic Lupus Erythematosus 16. Among its related pathways areRole of Calcineurin dependent NFAT signaling in lymphocytesandWnt Signaling Pathway and Pluripotency. Gene Ontology (GO) annotations related to this gene includetransferase activity, transferring phosphorus containing groupsandprotein tyrosine kinase activity. An important paralog of this gene isPRKCQ (PKC theta).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: nPKC-delta; nPKC-theta; PKC theta; Protein kinase C delta type; protein kinase C delta variant IX; protein kinase C delta VIII; Protein kinase C theta type; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C, theta; protein kinase C[d]; SDK-1; Tyrosine-protein kinase PRKCD

          View more View less

          Gene Aliases: A130035A12Rik; AI385711; ALPS3; AW494342; CVID9; D14Ertd420e; MAY1; nPKC-delta; nPKC-theta; PKC-0; PKC-theta; PKC[d]; PKCD; PKCdelta; Pkcq; PKCtheta; PRKCD; PRKCQ; PRKCT

          View more View less

          UniProt ID: (Human) Q05655, (Human) Q04759, (Mouse) P28867, (Mouse) Q02111

          View more View less

          Entrez Gene ID: (Human) 5580, (Human) 5588, (Mouse) 18753, (Mouse) 18761

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding enzyme activator activity enzyme binding kinase binding protein kinase binding insulin receptor substrate binding metal ion binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups TIR domain binding
          Process(es)
          stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process cellular component disassembly involved in execution phase of apoptosis cell cycle signal transduction activation of phospholipase C activity intrinsic apoptotic signaling pathway in response to oxidative stress regulation of receptor activity immunoglobulin mediated immune response histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation termination of signal transduction platelet activation negative regulation of actin filament polymerization positive regulation of endodeoxyribonuclease activity negative regulation of protein binding activation of protein kinase activity interleukin-10 production interleukin-12 production positive regulation of superoxide anion generation regulation of actin cytoskeleton organization negative regulation of glial cell apoptotic process positive regulation of protein dephosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis B cell proliferation neutrophil activation positive regulation of protein import into nucleus defense response to bacterium negative regulation of MAP kinase activity regulation of mRNA stability negative regulation of insulin receptor signaling pathway negative regulation of inflammatory response negative regulation of peptidyl-tyrosine phosphorylation protein stabilization negative regulation of filopodium assembly cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to hydrogen peroxide cellular response to hydroperoxide negative regulation of platelet aggregation cellular senescence positive regulation of phospholipid scramblase activity cellular response to angiotensin positive regulation of ceramide biosynthetic process positive regulation of glucosylceramide catabolic process positive regulation of sphingomyelin catabolic process positive regulation of response to DNA damage stimulus positive regulation of apoptotic signaling pathway response to oxidative stress aspartate transport phosphorylation collagen metabolic process cellular response to oxidative stress intracellular signal transduction regulation of phosphorylation positive regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of glucose import protein autophosphorylation regulation of cell growth response to hypoxia regulation of transcription, DNA-templated membrane protein ectodomain proteolysis inflammatory response axon guidance aging response to heat response to glucose regulation of G2/M transition of mitotic cell cycle response to organic cyclic compound regulation of vasoconstriction positive regulation of telomere maintenance via telomerase positive regulation of interleukin-17 production positive regulation of interleukin-4 production response to insulin Fc-epsilon receptor signaling pathway positive regulation of T cell proliferation tissue regeneration positive regulation of NF-kappaB import into nucleus positive regulation of interleukin-2 biosynthetic process respiratory burst positive regulation of protein secretion T cell receptor signaling pathway positive regulation of T cell activation positive regulation of NF-kappaB transcription factor activity positive regulation of filopodium assembly positive regulation of stress fiber assembly positive regulation of telomerase activity negative regulation of T cell apoptotic process regulation of platelet aggregation positive regulation of telomere capping positive regulation of T-helper 17 type immune response positive regulation of T-helper 2 cell activation immune system process
          It has to be done as per old AB suggested Products section.
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