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Invitrogen
Phospho-PLCG1 (Tyr775) Polyclonal Antibody
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Phospho-PLCG1 (Tyr775) Antibody (PA5-143744) in WB
Western blot of Phospho-PLCG1 (Tyr775) immunoprecipitation from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2,4,5,6). Immunoprecipitation was performed with PLCG1 monoclonal antibody (Product # MA5-47678). The blots were probed with anti-PLCgamma1 (lanes 1 & 2) and Phospho-PLCG1 (Tyr775) polyclonal antibody (Product # PA5-143744) (lanes 3-6). The latte... View More
Product Details
PA5-143744
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PLCgamma1 (Tyr-775) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 775 in human PLCgamma1. This sequence has high homology to the conserved site in rat and mouse PLCgamma1, and has low homology to PLCgamma2.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942972
Product Specific Information
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-PLCgamma1 (Tyr-775) peptide. The antibody detects a 150 kDa* protein in human Jurkat and A431 cells treated with pervanadate, but is not observed in untreated cells.
Target Information
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C2; PLC gamma1; PLC-148; PLC-gamma-1; PLC-II; PLC2I; triphosphoinositide phosphodiesterase
Gene Aliases: AI894140; Cded; NCKAP3; Plc-1; Plc-gamma1; PLC-II; PLC1; PLC148; Plcg-1; PLCG1; PLCgamma1; PPLCA
UniProt ID: (Human) P19174, (Mouse) Q62077, (Rat) P10686
Entrez Gene ID: (Human) 5335, (Mouse) 18803, (Rat) 25738
phosphatidylinositol phospholipase C activity
phospholipase C activity
receptor signaling protein activity
neurotrophin TRKA receptor binding
calcium ion binding
protein binding
protein kinase binding
receptor tyrosine kinase binding
glutamate receptor binding
signal transducer activity
insulin receptor binding
phosphoric diester hydrolase activity
hydrolase activity
metal ion binding
phosphoprotein binding
in utero embryonic development
lipid metabolic process
calcium ion transport
signal transduction
epidermal growth factor receptor signaling pathway
protein secretion
phospholipid catabolic process
positive regulation of epithelial cell migration
lipid catabolic process
cell migration
calcium-mediated signaling
inositol trisphosphate biosynthetic process
intracellular signal transduction
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
T cell receptor signaling pathway
positive regulation of release of sequestered calcium ion into cytosol
cellular response to epidermal growth factor stimulus
regulation of store-operated calcium channel activity
response to gravity
response to organonitrogen compound
response to hydrogen peroxide
response to morphine
activation of MAPKK activity
viral process
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
inositol phosphate metabolic process
leukocyte migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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