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Invitrogen
Phospho-PLCg1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (5I7E8)
FIGURE: 1 / 1
Phospho-PLCg1 (Tyr783) Antibody (MA5-55320) in WB
Western Blot analysis of various lysates using Phospho-PLCg1 (Tyr783) Recombinant Monoclonal Antibody (Product # MA5-55320) at 1:2,000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37&(8)451; for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk i... View More
Product Details
MA5-55320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5I7E8
Immunogen
A synthetic phosphorylated peptide around Y783 of human Phospho-PLC gamma 1.
Conjugate
Form
Liquid
Concentration
0.26 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667561
Product Specific Information
Immunogen Sequence: GFYVE
Target Information
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; cell differentiation and embryonic development; EC 3.1.4.11; OTTHUMP00000178982; Phosphoinositide phospholipase C-gamma-1; phospholipase C-1; Phospholipase C-gamma-1; Phospholipase C2; PLC gamma1; PLC-gamma-1; PLC2I
Gene Aliases: AI894140; Cded; Plc-1; Plc-gamma1; Plcg-1; Plcg1
UniProt ID: (Mouse) Q62077
Entrez Gene ID: (Mouse) 18803
phosphatidylinositol phospholipase C activity
signal transducer activity
insulin receptor binding
neurotrophin TRKA receptor binding
calcium ion binding
protein binding
phosphoric diester hydrolase activity
hydrolase activity
protein kinase binding
receptor tyrosine kinase binding
glutamate receptor binding
metal ion binding
phosphoprotein binding
in utero embryonic development
lipid metabolic process
calcium ion transport
signal transduction
epidermal growth factor receptor signaling pathway
protein secretion
phospholipid catabolic process
positive regulation of epithelial cell migration
lipid catabolic process
cell migration
calcium-mediated signaling
inositol trisphosphate biosynthetic process
intracellular signal transduction
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
T cell receptor signaling pathway
positive regulation of release of sequestered calcium ion into cytosol
cellular response to epidermal growth factor stimulus
regulation of store-operated calcium channel activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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