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Phospho-PLD2 (Tyr169) Antibody (PA5-36868) in WB
Western blot analysis of Phospho-PLD2 (Tyr169) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: C6 whole cell lysate (40 µg), Lane 3: the Embryo tissue lysate of mouse (40 µg), Lane 4: A549 whole cell lysate (40 µg), Lane 5: HeLa whole cell lysate (40 µg). Samples were incubated with Phospho-PLD2 (Tyr169) polyclonal antibody (Product # PA5-36868) at a dilution of 1:500.
Product Details
PA5-36868
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human PLD2 around the phosphorylation site of Tyrosine 169
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2553792
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 106 kDa.
Purity is >95% by SDS-PAGE.
Target Information
PLD2 is a peripheral membrane protein belonging to phospholipase D family with a PH domain, two PLD phosphodiesterase domains and a PX (phox homology) domain that plays a regulatory rather than catalytic role. PLD2 catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline. PLD2 may function in regulated secretion, signal-induced cytoskeletal regulation and/or endocytosis, transcriptional regulation, and cell cycle control. It is usually stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and activated by the ADP-ribosylation factor-1 (ARF-1). PLD2 is activated by agonist stimulation of both tyrosine kinase and G protein-coupled receptors and is also known to interact with EGFR and PIP5K1A. PLD2, localized to plasma membrane caveolae, is ubiquitously expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Choline phosphatase 2; hPLD2; Phosphatidylcholine-hydrolyzing phospholipase D2; phospholipase D gene 2; Phospholipase D2; PLD 2; PLD1C; rPLD2
Gene Aliases: PLD1C; PLD2; Pldc
UniProt ID: (Human) O14939, (Mouse) P97813, (Rat) P70498
Entrez Gene ID: (Human) 5338, (Mouse) 18806, (Rat) 25097
response to hypoxia
G-protein coupled receptor internalization
receptor-mediated endocytosis
phospholipid catabolic process
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
positive regulation of cell migration
neuron projection development
response to hydrogen peroxide
positive regulation of mast cell degranulation
response to peptide hormone
positive regulation of cell adhesion
positive regulation of receptor-mediated endocytosis
phosphatidic acid biosynthetic process
cytoskeleton organization
small GTPase mediated signal transduction
lipid catabolic process
synaptic vesicle recycling
Fc-gamma receptor signaling pathway involved in phagocytosis
inositol lipid-mediated signaling
cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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