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Invitrogen
Phospho-PLK1 (Ser482, Ser486, Ser490) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-PLK1 (Ser482, Ser486, Ser490) Antibody (PA1-4637) in WB
Western blot of PLK1 in rat hippocampal membrane lysate showing specific immunolabeling of a band at ~120 kDa corresponding to Phospho-PLK1 (Ser482, Ser486, Ser490) polyclonal antibody (Product # PA1-4637) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units overnight).
Product Details
PA1-4637
Product Specifications
Species Reactivity
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus PLK1 conjugated to KLH
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_560673
Product Specific Information
This antibody is predicted to react with rat, mouse, human and zebrafish based on 100% sequence homology.
This antibody is specific for the ~120 kDa PLKK protein phosphorylated at Ser482,486,490 in Western blots of Xenopus lysates.
Target Information
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PLK 1; PLK-1; Plx1; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase PLK1; STPK14
Gene Aliases: Plk; Plk1; plx1; XELAEV_18047692mg
UniProt ID: (Rat) Q62673, (Xenopus) P70032
Entrez Gene ID: (Rat) 25515, (Xenopus) 380481
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
mitotic cytokinesis
microtubule bundle formation
protein phosphorylation
mitotic nuclear division
mitotic spindle assembly checkpoint
regulation of mitotic cell cycle
positive regulation of peptidyl-threonine phosphorylation
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
regulation of mitotic metaphase/anaphase transition
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
polar body extrusion after meiotic divisions
negative regulation of apoptotic process
regulation of protein binding
homologous chromosome segregation
establishment of protein localization
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of proteolysis
response to antibiotic
centrosome organization
positive regulation of ubiquitin-protein transferase activity
protein localization to chromatin
positive regulation of ubiquitin protein ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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