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Invitrogen
Phospho-PPP1R12A/PPP1R12B/PPP1R12C (Thr696) Polyclonal Antibody
FIGURE: 1 / 1
Phospho-PPP1R12A/PPP1R12B/PPP1R12C (Thr696) Antibody (PA5-121344) in WB
Western blot analysis of Phospho-PPP1R12A/PPP1R12B/PPP1R12C (Thr696) in extracts of Jurkat cells. Samples were incubated with Phospho-PPP1R12A/PPP1R12B/PPP1R12C (Thr696) Polyclonal antibody (Product # PA5-121344) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after ... View More
Product Details
PA5-121344
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around T696 of human PPP1R12A/PPP1R12B/PPP1R12CPPP1R12A (NP_0024711)
Conjugate
Form
Liquid
Concentration
0.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914916
Product Specific Information
Positive test controls include: Jurkat.
Immunogen sequence: RSTQG
Target Information
Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. [NCBI]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: leukocyte receptor cluster (LRC) encoded novel gene 3; leukocyte receptor cluster (LRC) member 3; myosin binding subunit; Myosin phosphatase target subunit 1; Myosin phosphatase target subunit 2; myosin phosphatase, target subunit 1; myosin phosphatase, target subunit 2; Myosin phosphatase-targeting subunit 1; Myosin phosphatase-targeting subunit 2; myosin-binding subunit 85; PPP1R12C; Protein phosphatase 1 myosin-binding subunit of 85 kDa; Protein phosphatase 1 myosin-binding subunit p85; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase 1 regulatory subunit 12B; Protein phosphatase 1 regulatory subunit 12C; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12B; protein phosphatase 1, regulatory (inhibitor) subunit 12C; protein phosphatase 1, regulatory subunit 12A; protein phosphatase 1, regulatory subunit 12B; protein phosphatase 1, regulatory subunit 12C; Protein phosphatase myosin-binding subunit
Gene Aliases: LENG3; M130; MBS; MBS85; MYPT1; MYPT2; p84; p85; PP1bp55; PPP1R12A; PPP1R12B; PPP1R12C
UniProt ID: (Human) O14974, (Human) O60237, (Human) Q9BZL4
Entrez Gene ID: (Human) 4659, (Human) 4660, (Human) 54776
G2/M transition of mitotic cell cycle
protein dephosphorylation
mitotic nuclear division
signal transduction
regulation of cell adhesion
regulation of myosin-light-chain-phosphatase activity
positive regulation of myosin-light-chain-phosphatase activity
cellular response to drug
negative regulation of catalytic activity
positive regulation of transcription from RNA polymerase II promoter
regulation of nucleocytoplasmic transport
centrosome organization
regulation of establishment of endothelial barrier
regulation of muscle contraction
positive regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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