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Invitrogen
Phospho-Profilin 1 (Tyr129) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-Profilin 1 (Tyr129) Antibody (PA5-143753) in WB
Western blot of Phospho-Profilin 1 (Tyr129) in HUVEC stimulated with Pervanadate (1 mM) for 30 minutes (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or Phospho-Profilin 1 (Tyr129) polyclonal antibody (Product # PA5-143753) (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & ... View More
Product Details
PA5-143753
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Profilin (Tyr129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr129 in human Profilin-1. This sequence is conserved in rat and mouse Profilin-1. The tyrosine site has some homology to the conserved site (Tyr129) in Profilin-2a and Profilin-2b, the site is not conserved in Profiin-3 and Profilin-4.
Conjugate
Form
Liquid
Concentration
~1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942981
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated Profilin (Tyr-129) then affinity purified using Profilin (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.
Target Information
Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin binding protein; Epididymis tissue protein Li 184a; Profilin I; Profilin-1
Gene Aliases: ALS18; Pfn; PFN1
UniProt ID: (Human) P07737, (Rat) P62963, (Mouse) P62962
Entrez Gene ID: (Human) 5216, (Rat) 64303, (Mouse) 18643
neural tube closure
regulation of transcription from RNA polymerase II promoter
actin polymerization or depolymerization
response to organic substance
positive regulation of epithelial cell migration
negative regulation of actin filament polymerization
positive regulation of actin filament polymerization
negative regulation of actin filament bundle assembly
positive regulation of ATPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
positive regulation of DNA metabolic process
positive regulation of stress fiber assembly
negative regulation of stress fiber assembly
cellular response to growth factor stimulus
positive regulation of ruffle assembly
actin cytoskeleton organization
positive regulation of actin filament bundle assembly
protein stabilization
Wnt signaling pathway, planar cell polarity pathway
cell-cell adhesion
regulation of actin polymerization or depolymerization
sequestering of actin monomers
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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