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          • Primary Antibodies ›
          • PYK2 Antibodies

          Bioss

          Phospho-Pyk2 (Tyr402) Polyclonal Antibody

          View all (39) PYK2 antibodies

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          Datasheet
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          Cite Phospho-Pyk2 (Tyr402) Polyclonal Antibody

          • Antibody Testing Data (2)
          Phospho-Pyk2 (Tyr402) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-Pyk2 (Tyr402) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-Pyk2 (Tyr402) Antibody (BS-3400R) in IHC (P)

          Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with
          Pyk2 (Tyr402) Polyclonal Antibody (bs-3400R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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          Phospho-Pyk2 (Tyr402) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-Pyk2 (Tyr402) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Product Details

          BS-3400R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:400
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic phosphopeptide derived from human Pyk2/PTK2B around the phosphorylation site of Tyr402.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. PTK2B regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. FAK2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors, cytokine, chemokine, and growth factor receptors, and mediates responses to cellular stress. Aberrant FAK2 expression may play a role in cancer cell proliferation, migration, and invasion in tumor formation and metastasis. Elevated expression has been seen in gliomas, hepatocellular carcinoma, lung cancer, and breast cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: CADTK; CAK beta; CAK-beta; Calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; cellular adhesion kinase beta; FADK; FADK 2; FAK; FAK1; Focal adhesion kinase 2; FRNK; pp125FAK; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; Protein-tyrosine kinase 2-beta; PTK2 protein tyrosine kinase 2 beta; PTK2B protein tyrosine kinase 2 beta; RAFTK; Related adhesion focal tyrosine kinase

          View more View less

          Gene Aliases: CADTK; CAKB; CAKbeta; E430023O05Rik; FADK2; FAK2; PKB; PTK; PTK2B; PYK2; RAFTK

          View more View less

          UniProt ID: (Human) Q14289, (Rat) P70600, (Mouse) Q9QVP9

          View more View less

          Entrez Gene ID: (Human) 2185, (Rat) 50646, (Mouse) 19229

          View more View less

          Function(s)
          calmodulin-dependent protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity NMDA glutamate receptor activity receptor binding protein binding ATP binding protein complex binding 3-phosphoinositide-dependent protein kinase binding protein kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
          Process(es)
          MAPK cascade response to reactive oxygen species angiogenesis oocyte maturation response to hypoxia positive regulation of cell-matrix adhesion sprouting angiogenesis marginal zone B cell differentiation protein phosphorylation cellular defense response response to osmotic stress actin filament organization cell adhesion cell surface receptor signaling pathway signal complex assembly epidermal growth factor receptor signaling pathway positive regulation of cytosolic calcium ion concentration integrin-mediated signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to mechanical stimulus response to hormone response to glucose response to lithium ion response to organonitrogen compound positive regulation of endothelial cell migration negative regulation of muscle cell apoptotic process regulation of cGMP-mediated signaling regulation of macrophage chemotaxis positive regulation of neuron projection development glial cell proliferation peptidyl-tyrosine phosphorylation cell differentiation regulation of cell adhesion negative regulation of ossification positive regulation of cell growth positive regulation of cell migration negative regulation of bone mineralization regulation of cGMP biosynthetic process positive regulation of actin filament polymerization neuron projection development regulation of inositol trisphosphate biosynthetic process tumor necrosis factor-mediated signaling pathway ionotropic glutamate receptor signaling pathway response to immobilization stress peptidyl-tyrosine autophosphorylation response to cocaine response to drug response to hydrogen peroxide activation of Janus kinase activity negative regulation of apoptotic process stress fiber assembly negative regulation of potassium ion transport positive regulation of JUN kinase activity negative regulation of neuron apoptotic process blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of nitric oxide biosynthetic process bone resorption response to ethanol negative regulation of myeloid cell differentiation positive regulation of translation positive regulation of angiogenesis positive regulation of protein kinase activity positive regulation of JNK cascade vascular endothelial growth factor receptor signaling pathway focal adhesion assembly positive regulation of peptidyl-tyrosine phosphorylation regulation of calcium-mediated signaling positive regulation of nitric-oxide synthase activity regulation of release of sequestered calcium ion into cytosol response to cAMP response to calcium ion positive regulation of synaptic transmission, glutamatergic long-term synaptic potentiation chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to retinoic acid cellular response to fluid shear stress activation of GTPase activity regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of establishment of cell polarity regulation of actin cytoskeleton reorganization regulation of N-methyl-D-aspartate selective glutamate receptor activity positive regulation of reactive oxygen species metabolic process positive regulation of excitatory postsynaptic potential positive regulation of B cell chemotaxis adaptive immune response protein complex assembly apoptotic process response to stress signal transduction positive regulation of nitric oxide biosynthetic process protein autophosphorylation long term synaptic depression positive regulation of DNA biosynthetic process immune system process cytoskeleton organization phosphorylation cell migration
          It has to be done as per old AB suggested Products section.
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