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          • Primary Antibodies ›
          • RAC1/CDC42 Antibodies

          Invitrogen

          Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody

          View all (6) RAC1/CDC42 antibodies

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          Cite Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody

          • Antibody Testing Data (4)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-RAC1/CDC42 (Ser71) Antibody (PA5-85105) in ICC/IF

          Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody detects Rac1 protein at cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rac1 stained by Phospho-RAC1/CDC42 (Ser71) Polyclonal Antibody (Product # PA5-85105) diluted at 1:500. Blue: Fluoroshield with DAPI . {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-RAC1/CDC42 (Ser71) Antibody in Western Blot (WB)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Western Blot (WB)

          Product Details

          PA5-85105

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human Rac1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.79 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792253

          Product Specific Information

          Keep as concentrated solution.

          Predicted reactivity: Mouse (97%), Rat (90%), Drosophila (82%), Xenopus laevis (90%), Dog (90%), Chicken (90%), Sheep (90%), Chimpanzee (90%), Bovine (90%).

          Positive Control: 293T, A431, HeLa, HepG2, Molt-4, Raji, mouse liver.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; Cell migration-inducing gene 5 protein; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; p21-Rac1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); rho family, small GTP binding protein Rac1; small GTP binding protein CDC42

          View more View less

          Gene Aliases: AI747189; AL023026; AU018915; CDC42; CDC42Hs; D5Ertd559e; G25K; MIG5; p21-Rac1; Rac; Rac-1; RAC1; TC-25; TC25; TKS

          View more View less

          UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P60766, (Human) P63000, (Mouse) P63001, (Rat) Q6RUV5

          View more View less

          Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540, (Human) 5879, (Mouse) 19353, (Rat) 363875

          View more View less

          Function(s)
          GTPase activity protein binding GTP binding protein kinase binding GTP-dependent protein binding mitogen-activated protein kinase kinase kinase binding thioesterase binding apolipoprotein A-I receptor binding identical protein binding Rho GDP-dissociation inhibitor binding ubiquitin protein ligase activity nucleotide binding Rab GTPase binding enzyme binding histone deacetylase binding
          Process(es)
          positive regulation of protein phosphorylation sprouting angiogenesis cardiac conduction system development keratinocyte development endocytosis actin filament organization Golgi organization regulation of mitotic nuclear division nuclear migration small GTPase mediated signal transduction metabolic process response to glucose positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion regulation of exocytosis substantia nigra development establishment of cell polarity actin cytoskeleton organization hair follicle morphogenesis positive regulation of pseudopodium assembly negative regulation of protein complex assembly keratinization regulation of protein stability positive regulation of cytokinesis positive regulation of peptidyl-serine phosphorylation adherens junction organization cellular protein localization establishment or maintenance of apical/basal cell polarity multicellular organism growth dendritic cell migration regulation of protein catabolic process positive regulation of catalytic activity positive regulation of MAPK cascade regulation of protein heterodimerization activity positive regulation of neuron apoptotic process positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication regulation of protein kinase activity positive regulation of JNK cascade filopodium assembly neuron fate determination epidermis morphogenesis actin filament bundle assembly regulation of protein metabolic process regulation of filopodium assembly nucleus localization establishment of Golgi localization positive regulation of synapse structural plasticity regulation of attachment of spindle microtubules to kinetochore heart contraction canonical Wnt signaling pathway submandibular salivary gland formation epithelial-mesenchymal cell signaling hair follicle placode formation positive regulation of hair follicle cell proliferation organelle transport along microtubule actin filament branching epithelial cell-cell adhesion positive regulation of intracellular protein transport positive regulation of substrate adhesion-dependent cell spreading auditory receptor cell morphogenesis mast cell chemotaxis epithelial cell morphogenesis phagocytosis, engulfment chemotaxis hyperosmotic response cytoskeleton organization cell adhesion G-protein coupled receptor signaling pathway axon guidance cell proliferation positive regulation of lamellipodium assembly regulation of fibroblast migration positive regulation of cell-substrate adhesion regulation of neuron maturation dendrite development cell migration cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development regulation of cell morphogenesis lamellipodium assembly actin filament polymerization regulation of cell migration positive regulation of actin filament polymerization ruffle organization negative regulation of interleukin-23 production substrate adhesion-dependent cell spreading engulfment of apoptotic cell cell-cell junction organization bone resorption anatomical structure arrangement dendrite morphogenesis cell motility homeostasis of number of cells within a tissue positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly synaptic transmission, GABAergic Wnt signaling pathway, planar cell polarity pathway regulation of respiratory burst cellular response to mechanical stimulus semaphorin-plexin signaling pathway dopaminergic neuron differentiation protein localization to plasma membrane positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly establishment or maintenance of cell polarity blood coagulation protein ubiquitination macrophage differentiation positive regulation of cell growth T cell costimulation Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of epidermal growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway regulation of small GTPase mediated signal transduction positive regulation of muscle cell differentiation positive regulation of epithelial cell proliferation involved in lung morphogenesis movement of cell or subcellular component inflammatory response cell-matrix adhesion regulation of cell size response to wounding anatomical structure morphogenesis regulation of hydrogen peroxide metabolic process platelet activation positive regulation of Rho protein signal transduction intracellular signal transduction Fc-epsilon receptor signaling pathway positive regulation of apoptotic process negative regulation of receptor-mediated endocytosis regulation of defense response to virus by virus Rho protein signal transduction nervous system development endosomal transport cell differentiation Rac protein signal transduction
          It has to be done as per old AB suggested Products section.
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