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          • Primary Antibodies ›
          • RAC1/CDC42 Antibodies

          Invitrogen

          Phospho-RAC1/CDC42 (Ser71) Recombinant Rabbit Monoclonal Antibody (20H14L7)

          Advanced Verification
          View all (6) RAC1/CDC42 antibodies

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          Datasheet
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          Cite Phospho-RAC1/CDC42 (Ser71) Recombinant Rabbit Monoclonal Antibody (20H14L7)

          • Antibody Testing Data (3)
          • Advanced Verification (3)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Phospho-RAC1/CDC42 (Ser71) Antibody (701942) in ICC/IF

          For immunofluorescence analysis A431 cells were fixed and permeabilized for detection of Phospho-Rac1/Cdc42 (pS71) using Phospho-Rac1/Cdc42pS71 Recombinant Rabbit Monoclonal Antibody (Product # 701942, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-RAC1/CDC42 (Ser71) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Western Blot (WB)
          Phospho-RAC1/CDC42 (Ser71) Antibody in Western Blot (WB)
          Phospho-RAC1/CDC42 (Ser71) Antibody
          Phospho-RAC1/CDC42 (Ser71) Antibody
          Phospho-RAC1/CDC42 (Ser71) Antibody

          Product Details

          701942

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          20H14L7

          Immunogen

          Peptide corresponding to Human CDC42 (aa 68-76)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2608404

          Product Specific Information

          This antibody is predicted to react with Pig, Rat, Monkey and Mouse.

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Cell division control protein 42 homolog; Cell migration-inducing gene 5 protein; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; p21-Rac1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; small GTP binding protein CDC42

          View more View less

          Gene Aliases: CDC42; CDC42Hs; G25K; MIG5; p21-Rac1; Rac-1; RAC1; TC-25; TC25; TKS

          View more View less

          UniProt ID: (Human) P60953, (Human) P63000

          View more View less

          Entrez Gene ID: (Human) 998, (Human) 5879

          View more View less

          Function(s)
          GTPase activity protein binding GTP binding protein kinase binding GTP-dependent protein binding mitogen-activated protein kinase kinase kinase binding thioesterase binding apolipoprotein A-I receptor binding identical protein binding Rho GDP-dissociation inhibitor binding ubiquitin protein ligase activity Rab GTPase binding enzyme binding histone deacetylase binding
          Process(es)
          sprouting angiogenesis cardiac conduction system development keratinocyte development Golgi organization regulation of mitotic nuclear division nuclear migration establishment or maintenance of cell polarity small GTPase mediated signal transduction blood coagulation positive regulation of gene expression negative regulation of gene expression protein ubiquitination substantia nigra development actin cytoskeleton organization macrophage differentiation positive regulation of cell growth hair follicle morphogenesis positive regulation of pseudopodium assembly T cell costimulation negative regulation of protein complex assembly keratinization regulation of protein stability positive regulation of cytokinesis positive regulation of peptidyl-serine phosphorylation adherens junction organization cellular protein localization establishment or maintenance of apical/basal cell polarity multicellular organism growth dendritic cell migration Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of epidermal growth factor receptor signaling pathway regulation of protein catabolic process regulation of protein heterodimerization activity positive regulation of neuron apoptotic process positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication regulation of protein kinase activity positive regulation of JNK cascade filopodium assembly vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway neuron fate determination actin filament bundle assembly regulation of small GTPase mediated signal transduction positive regulation of muscle cell differentiation regulation of filopodium assembly establishment of Golgi localization positive regulation of synapse structural plasticity regulation of attachment of spindle microtubules to kinetochore heart contraction canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway positive regulation of epithelial cell proliferation involved in lung morphogenesis submandibular salivary gland formation epithelial-mesenchymal cell signaling hair follicle placode formation positive regulation of hair follicle cell proliferation organelle transport along microtubule actin filament branching epithelial cell-cell adhesion positive regulation of intracellular protein transport positive regulation of substrate adhesion-dependent cell spreading positive regulation of protein phosphorylation auditory receptor cell morphogenesis mast cell chemotaxis epithelial cell morphogenesis movement of cell or subcellular component inflammatory response hyperosmotic response cell adhesion cell-matrix adhesion G-protein coupled receptor signaling pathway axon guidance cell proliferation regulation of cell size response to wounding anatomical structure morphogenesis regulation of hydrogen peroxide metabolic process positive regulation of lamellipodium assembly regulation of fibroblast migration positive regulation of cell-substrate adhesion regulation of neuron maturation cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development lamellipodium assembly actin filament polymerization platelet activation regulation of cell migration positive regulation of actin filament polymerization ruffle organization negative regulation of interleukin-23 production substrate adhesion-dependent cell spreading positive regulation of Rho protein signal transduction intracellular signal transduction Fc-epsilon receptor signaling pathway positive regulation of apoptotic process engulfment of apoptotic cell cell-cell junction organization bone resorption negative regulation of receptor-mediated endocytosis anatomical structure arrangement dendrite morphogenesis cell motility homeostasis of number of cells within a tissue regulation of defense response to virus by virus positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly synaptic transmission, GABAergic regulation of respiratory burst cellular response to mechanical stimulus semaphorin-plexin signaling pathway dopaminergic neuron differentiation protein localization to plasma membrane positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly
          It has to be done as per old AB suggested Products section.
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