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Phospho-RAD23A (Ser357) Antibody (PA5-118713) in ICC/IF
Immunocytochemistry analysis of Phospho-RAD23A (Ser357) in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-RAD23A (Ser357) polyclonal antibody (Product # PA5-118713) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an Al... View More
Product Details
PA5-118713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAD23A(Accession P54725), corresponding to amino acid residues around phosphorylated Ser357.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2903213
Product Specific Information
Antibody detects endogenous levels of RAD23 only when phosphorylated at Ser357.
Target Information
The product of this gene is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. In addition to the expressed gene at this chromosomal locus (11q22), there are pseudogenes located on chromosomes 20 and 21. This gene product has been identified in a number of different tissues but all forms have been shown to be identical and are not tissue specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HR23A; MGC111083; rad23; RAD23, yeast homolog, A; UV excision repair protein RAD23 homolog A
Gene Aliases: 2310040P19Rik; AL024030; HHR23A; HR23A; Mhr23a; RAD23A
UniProt ID: (Human) P54725, (Mouse) P54726
Entrez Gene ID: (Human) 5886, (Rat) 361381, (Mouse) 19358
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