Active Motif

Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (3E8)

Phospho-RNA pol II CTD (Ser5) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Phospho-RNA pol II CTD (Ser5) Antibody (61085) in ICC/IF

Detection of RNA Pol II pS5 by immunofluorescence U2OS cells were stained with RNA Pol II pS5 antibody at a dilution of 1:500. Left panel: RNA Pol II pS5 antibody staining. Middle panel:DAPI. Right panel: merge.

Phospho-RNA pol II CTD (Ser5) Antibody in Western Blot (WB)

Phospho-RNA pol II CTD (Ser5) Antibody in ChIP Assay (ChIP)

Phospho-RNA pol II CTD (Ser5) Antibody in ChIP-Sequencing (ChIP-Seq)

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Product Details

61085

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

Immunocytochemistry (ICC/IF)

1:500

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

5-10 µg

View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

5-10 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat, Yeast

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3E8

Immunogen

This RNA pol II CTD phospho Ser5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

70mM tris, pH 8, with 30% glycerol, 31mM glycine, 105mM NaCl, 0.07mM EDTA

Contains

0.035% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2687451

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Amyloid-like Assembly Activates a Phosphatase in the Developing Drosophila Embryo.

Cell

Nil Z,Hervás R,Gerbich T,Leal P,Yu Z,Saraf A,Sardiu M,Lange JJ,Yi K,Unruh J,Slaughter B,Si K

Research topic: Embryology, Developmental Biology

Thu Sep 05 00:00:00 UTC 2019

Ring finger protein 121 is a potent regulator of adeno-associated viral genome transcription.

PLoS pathogens

Madigan VJ,Yuziuk JA,Chiarella AM,Tyson TO,Meganck RM,Elmore ZC,Tse LV,Hathaway NA,Asokan A

Research topic: Transcription

Thu Aug 01 00:00:00 UTC 2019

NF-κB inhibition rescues cardiac function by remodeling calcium genes in a Duchenne muscular dystrophy model.

Nature communications

Peterson JM,Wang DJ,Shettigar V,Roof SR,Canan BD,Bakkar N,Shintaku J,Gu JM,Little SC,Ratnam NM,Londhe P,Lu L,Gaw CE,Petrosino JM,Liyanarachchi S,Wang H,Janssen PML,Davis JP,Ziolo MT,Sharma SM,Guttridge DC

Research topic: Transcription

Fri Aug 24 00:00:00 UTC 2018

Real-time observation of light-controlled transcription in living cells.

Journal of cell science

Rademacher A,Erdel F,Trojanowski J,Schumacher S,Rippe K

Research topic: Gene Regulation

Fri Dec 15 00:00:00 UTC 2017

Acetylation on histone H3 lysine 9 mediates a switch from transcription initiation to elongation.

The Journal of biological chemistry

Gates LA,Shi J,Rohira AD,Feng Q,Zhu B,Bedford MT,Sagum CA,Jung SY,Qin J,Tsai MJ,Tsai SY,Li W,Foulds CE,O'Malley BW

Fri Sep 01 00:00:00 UTC 2017

Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription of Arc/Arg3.1 and Other Immediate Early Genes.

eNeuro

Dunn CJ,Sarkar P,Bailey ER,Farris S,Zhao M,Ward JM,Dudek SM,Saha RN

Research topic: Brain & Nerves

Mon Sep 17 00:00:00 UTC 2018

Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue.

Cell reports

Harlen KM,Trotta KL,Smith EE,Mosaheb MM,Fuchs SM,Churchman LS

Research topic: Transcription Regulation

Tue Jun 07 00:00:00 UTC 2016

Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts.

Molecular and cellular biology

Eifler TT,Shao W,Bartholomeeusen K,Fujinaga K,Jäger S,Johnson JR,Luo Z,Krogan NJ,Peterlin BM

Research topic: Transcription Regulation

Thu Jan 01 00:00:00 UTC 2015

DNA demethylation by 5-aza-2'-deoxycytidine is imprinted, targeted to euchromatin, and has limited transcriptional consequences.

Epigenetics & chromatin

Ramos MP,Wijetunga NA,McLellan AS,Suzuki M,Greally JM

Research topic: Transcription Regulation

Wed Mar 25 00:00:00 UTC 2015

Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis.

Cell stem cell

Hammoud SS,Low DH,Yi C,Carrell DT,Guccione E,Cairns BR

Research topic: Spermatogenesis, Stem Cells

Thu Aug 07 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8

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Gene Aliases: hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

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UniProt ID: (Human) P24928

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Entrez Gene ID: (Human) 5430

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Function(s)

RNA polymerase II activity DNA binding DNA-directed RNA polymerase activity RNA-directed RNA polymerase activity protein binding ubiquitin protein ligase binding poly(A) RNA binding metal ion binding

Process(es)

mRNA splicing, via spliceosome transcription, RNA-templated transcription-coupled nucleotide-excision repair DNA-templated transcription, termination regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA positive regulation of RNA splicing somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (3E8)

Phospho-RNA pol II CTD (Ser5) Antibody (61085) in ICC/IF

Product Details

61085

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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