Invitrogen

Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

Phospho-RNA pol II CTD (Ser5) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-RNA pol II CTD (Ser5) Antibody (MA1-46093) in ICC/IF

Immunofluorescence analysis of NPhospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8) was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8) (Pr... View More

Phospho-RNA pol II CTD (Ser5) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-RNA pol II CTD (Ser5) Antibody in Western Blot (WB)

Phospho-RNA pol II CTD (Ser5) Antibody in Immunoprecipitation (IP)

Phospho-RNA pol II CTD (Ser5) Antibody in ChIP Assay (ChIP)

Phospho-RNA pol II CTD (Ser5) Antibody in RNA Immunoprecipitation (RIP)

Phospho-RNA pol II CTD (Ser5) Antibody in CUT&RUN (C&R)

Phospho-RNA pol II CTD (Ser5) Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

MA1-46093

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:50 - 1:100

ELISA (ELISA)

1:100-1:2,000

Immunoprecipitation (IP)

1-5 µg

View 2 publications 2 publications

ChIP assay (ChIP)

1-10 µL

View 4 publications 4 publications

RNA Immunoprecipitation (RIP)

Assay-dependent

CUT&RUN (C&R)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Yeast

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4H8

Immunogen

Chemically synthesized phospho-ser 5 YSPTSpPS (Human).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1018366

Product Specific Information

MA1-46093 reacts with DNA-directed RNA Polymerase II Largest Subunit in human, yeast and mouse samples. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology.

MA1-46093 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, IHC (P) and Immunoprecipitation applications.

The MA1-46093 immunogen is 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). This antibody detects both the phosphorylated and non-phosphorylated forms.

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ANP32A represses Wnt signaling across tissues thereby protecting against osteoarthritis and heart disease.

Osteoarthritis and cartilage

Monteagudo S,Cornelis FMF,Wang X,de Roover A,Peeters T,Quintiens J,Sermon A,de Almeida RC,Meulenbelt I,Lories RJ

MA1-46093 was used in ChIP assay to assess Wnt target genes, differentiation markers, and matrix deposition. The research involved quantifying Wnt target genes in Anp32a-deficient mice and human chondrocytes after ANP32A silencing. The results highlight ANP32A as a significant repressor of Wnt signaling with implications for osteoarthritis and cardiac conditions.

Sun May 01 00:00:00 UTC 2022

Glucocorticoid signaling induces transcriptional memory and universally reversible chromatin changes.

Life science alliance

Bothe M,Buschow R,Meijsing SH

MA1-46093 was used in Chromatin immunoprecipitation to find that the hormone-induced changes were universally reversed after 1 d.

Fri Oct 01 00:00:00 UTC 2021

Telomerase Reverse Transcriptase Preserves Neuron Survival and Cognition in Alzheimer's Disease Models.

Nature aging

Shim HS,Horner JW,Wu CJ,Li J,Lan ZD,Jiang S,Xu X,Hsu WH,Zal T,Flores II,Deng P,Lin YT,Tsai LH,Wang YA,DePinho RA

MA1-46093 was used in Immunoprecipitation to provide genetic proof-of-concept for somatic TERT gene activation therapy in attenuating AD progression including cognitive decline.

Wed Dec 01 00:00:00 UTC 2021

TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors.

Cancers

Mert U,Adawy A,Scharff E,Teichmann P,Willms A,Haselmann V,Colmorgen C,Lemke J,von Karstedt S,Fritsch J,Trauzold A

MA1-46093 was used in Western Blotting to highlight the novel role for surface-activated TRAIL-Rs by direct trafficking and signaling into the nucleus, a previously unknown signaling principle for cell surface receptors that belong to the TNF-superfamily.

Wed Aug 14 00:00:00 UTC 2019

Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates.

Cell discovery

Zhang P,Chen Z,Li R,Guo Y,Shi H,Bai J,Yang H,Sheng M,Li Z,Li Z,Li J,Chen S,Yuan W,Cheng T,Xu M,Zhou Y,Yang FC

Published figure using POLR2A monoclonal antibody (Product # MA1-46093) in Immunoprecipitation

Thu Oct 01 00:00:00 UTC 2020

Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates.

Cell discovery

Zhang P,Chen Z,Li R,Guo Y,Shi H,Bai J,Yang H,Sheng M,Li Z,Li Z,Li J,Chen S,Yuan W,Cheng T,Xu M,Zhou Y,Yang FC

Published figure using POLR2A monoclonal antibody (Product # MA1-46093) in Immunoprecipitation

Thu Oct 01 00:00:00 UTC 2020

p53 suppresses muscle differentiation at the myogenin step in response to genotoxic stress.

Cell death and differentiation

Yang ZJ,Broz DK,Noderer WL,Ferreira JP,Overton KW,Spencer SL,Meyer T,Tapscott SJ,Attardi LD,Wang CL

MA1-46093 was used in Chromatin immunoprecipitation to reveal a mechanistic link between p53 and muscle differentiation.

Wed Apr 01 00:00:00 UTC 2015

p53 suppresses muscle differentiation at the myogenin step in response to genotoxic stress.

Cell death and differentiation

Yang ZJ,Broz DK,Noderer WL,Ferreira JP,Overton KW,Spencer SL,Meyer T,Tapscott SJ,Attardi LD,Wang CL

MA1-46093 was used in Chromatin immunoprecipitation to reveal a mechanistic link between p53 and muscle differentiation.

Wed Apr 01 00:00:00 UTC 2015

Hepatitis C virus infection activates an innate pathway involving IKK-α in lipogenesis and viral assembly.

Nature medicine

Li Q,Pène V,Krishnamurthy S,Cha H,Liang TJ

MA1-46093 was used in ChIP assay to study the mechaism by which HCV infection activates IKK-alpha and stimulates lipogenesis and lipid droplet formation

Sat Jun 01 00:00:00 UTC 2013

Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.

DNA repair

Zhu Q,Wani G,Sharma N,Wani A

MA1-46093 was used in western blot to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair

Sat Dec 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 3'-5' exodeoxyribonuclease; 3'-5' exoribonuclease; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; Pol2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8

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Gene Aliases: 220kDa; DUN2; hRPB220; hsRPB1; N0825; POL2; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2; YNL262W

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UniProt ID: (Baker's yeast) P21951, (Human) P24928, (Mouse) P08775

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Entrez Gene ID: (Baker's yeast) 855459, (Human) 5430, (Mouse) 20020

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Function(s)

nucleotide binding nucleic acid binding DNA binding double-stranded DNA binding single-stranded DNA binding DNA-directed DNA polymerase activity zinc ion binding single-stranded DNA 3'-5' exodeoxyribonuclease activity transferase activity nucleotidyltransferase activity metal ion binding iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding RNA polymerase II activity DNA-directed RNA polymerase activity RNA-directed RNA polymerase activity protein binding ubiquitin protein ligase binding poly(A) RNA binding core promoter binding

Process(es)

DNA synthesis involved in DNA repair nucleobase-containing compound metabolic process DNA replication DNA-dependent DNA replication leading strand elongation base-excision repair nucleotide-excision repair, DNA gap filling double-strand break repair via nonhomologous end joining mitotic sister chromatid cohesion intra-S DNA damage checkpoint mitotic DNA replication checkpoint gene conversion error-prone translesion synthesis DNA replication proofreading heterochromatin organization involved in chromatin silencing transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of RNA splicing mRNA splicing, via spliceosome transcription, RNA-templated transcription-coupled nucleotide-excision repair DNA-templated transcription, termination regulation of transcription, DNA-templated transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

Phospho-RNA pol II CTD (Ser5) Antibody (MA1-46093) in ICC/IF

Product Details

MA1-46093

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

CUT&RUN (C&R)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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