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Phospho-ROS1 (Tyr2274) Antibody (PA5-105915) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-ROS1 (Tyr2274) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-ROS1 (Tyr2274) polyclonal antibody (Product # PA5-105915) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105915
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ROS1(Accession P08922), corresponding to amino acid residues around phosphorylated Tyr2274.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2817314
Product Specific Information
Antibody detects endogenous levels of ROS only when phosphorylated at Tyr2274.
Target Information
ROS1 is a tyrosine kinase that functions as a growth or differentiation factor receptor. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-ros oncogene 1 , receptor tyrosine kinase; c-Ros receptor tyrosine kinase; c-ros-1; heart - derived c - ros - 1 proto - oncogene; Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1; ROS proto-oncogene 1 , receptor tyrosine kinase; Ros1 proto-oncogene; RP1-179P9.1; transmembrane tyrosine-specific protein kinase; v-ros avian UR2 sarcoma virus oncogene homolog 1; v-ros UR2 sarcoma virus oncogene homolog 1
Gene Aliases: c-ros; c-ros-1; MCF3; ROS; Ros-1; ROS1; ROS1C
UniProt ID: (Human) P08922, (Rat) Q63132, (Mouse) Q78DX7
Entrez Gene ID: (Human) 6098, (Rat) 25346, (Mouse) 19886
columnar/cuboidal epithelial cell development
protein phosphorylation
spermatogenesis
cell proliferation
negative regulation of gene expression
regulation of phosphate transport
cell growth
peptidyl-tyrosine phosphorylation
signal transduction by protein phosphorylation
cell differentiation
regulation of TOR signaling
peptidyl-tyrosine autophosphorylation
regulation of ERK1 and ERK2 cascade
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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