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Phospho-RSK1 (Ser363) Antibody (PA5-97389) in WB
Western blot analysis of Phospho-RSK1 (Ser363) in extracts of various cell lines. Samples were incubated with Phospho-RSK1 (Ser363) Polyclonal antibody (Product # PA5-97389) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins:... View More
Product Details
PA5-97389
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S363 of human p90Rsk/RSK1/RPS6KA1 (NP_0029442)
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809191
Product Specific Information
Positive Samples: HeLa, C6; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: KDSPG
Target Information
RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; p90-RSK 1; p90RSK1; p90S6K; pp90RSK1; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK-1; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1
Gene Aliases: HU-1; MAPKAPK1A; p90Rsk; RPS6KA1; RSK; RSK1
UniProt ID: (Human) Q15418, (Rat) Q63531
Entrez Gene ID: (Human) 6195, (Rat) 81771
protein phosphorylation
apoptotic process
cell cycle
signal transduction
positive regulation of cell growth
intracellular signal transduction
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of translation in response to stress
regulation of DNA-templated transcription in response to stress
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte proliferation
positive regulation of hepatic stellate cell activation
positive regulation of transcription, DNA-templated
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