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Invitrogen
Phospho-S6 (Ser244) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-S6 (Ser244) Antibody (PA5-143636) in WB
Western blot of Phospho-S6 (Ser244) in Jurkat cell lysate showing specific immunolabeling at ~28 kDa for Phospho-S6 (Ser244) polyclonal antibody (Product # PA5-143636) in the first lane (-). Phospho specificity is shown in the second lane (+) where immunolabeling is blocked by pre-adsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Product Details
PA5-143636
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 of mouse rpS6, conjugated to keyhole limpet hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 150mM NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942864
Product Specific Information
Specific for endogenous levels of the ~28 kDa rpS6 protein phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6
Gene Aliases: Rps6; S6R
UniProt ID: (Mouse) P62754
Entrez Gene ID: (Mouse) 20104
G1/S transition of mitotic cell cycle
placenta development
T cell proliferation involved in immune response
rRNA processing
translation
activation-induced cell death of T cells
mitotic nuclear division
mitotic cell cycle checkpoint
gastrulation
oogenesis stage
TOR signaling
T cell differentiation in thymus
ribosomal small subunit biogenesis
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
erythrocyte development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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